MAPK Pathway Phospho Antibody Array features 185 high-specificity antibodies targeting key nodes of the MAPK signaling pathway, optimized for protein phosphorylation profiling in human, mouse, and rat cell and tissue samples. Each kit includes two identical array slides, enabling parallel analysis of two independent samples, such as a control specimen and a treated experimental sample.
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Product Property | |
|---|---|
| Species Reactivity | Human; Mouse; Rat |
| Sample Type | Cell lysate; Tissue lysate |
| Assay Type | Fluorescent Immunoassay |
| Detection Method | Fluorescence Scanning |
| Internal Controls | Beta-actin; GAPDH; Negative controls |
| Principle | Fluorescent immunoassay based on antibody microarray, for quantitative detection of MAPK pathway phosphorylation levels. |
| Platform | Fluorescent Antibody Microarray |
| Specificity | Human |
| Cross-Reaction | Mouse; Rat |
| Slide Dimensions | 76 x 25 x 1 mm |
| Application Notes | ELISA based antibody array |
| Core Highlights | Site-specific phosphorylation profiling and screening with 185 antibodies Antibodies covalently immobilized on 3D polymer coated glass slide Fluorescent detection |
| Signaling Pathway | MAPK Signaling Pathway |
| Storage | 2-8°C |
Target Information | |
|---|---|
| Analyte | MAPK Pathway Phosphoproteins |
| Targets | 4E-BP1(Thr36), 4E-BP1(Thr45), ASK1(Ser83), ASK1(Ser966), ATF2(Ser112 or 94), ATF2(Ser62 or 44), ATF2(Thr69 or 51), ATF2(Thr71 or 53), ATF2(Thr73 or 55), ATF4(Ser245), c-Jun(Ser243), c-Jun(Ser63), c-Jun(Ser73), c-Jun(Thr239), c-Jun(Thr91), c-Jun(Thr93), c-Jun(Tyr170), c-Kit(Tyr721), CREB(Ser129), CREB(Ser133), eIF2 alpha(Ser51), elF4E(Ser209), Elk1(Ser383), Elk1(Ser389), Elk1(Thr417), Estrogen Receptor-alpha (Ser104), Estrogen Receptor-alpha (Ser106), Estrogen Receptor-alpha (Ser118), Estrogen Receptor-alpha (Ser167), FAK(Tyr861), FAK(Tyr925), Histone H2A.X(Ser139), Histone H3.1(Ser10), HSF1(Ser303), HSP27(Ser15), HSP27(Ser78), HSP27(Ser82), IRS-1(Ser307), IRS-1(Ser312), IRS-1(Ser636), IRS-1(Ser639), JunB(Ser259), JunB(Ser79), JunD(Ser255), MEF2A(Thr312), MEF2A(Thr319), MEK1(Ser217), MEK1(Ser221), MEK1(Thr291), MEK2(Thr394), Met(Tyr1234), Met(Tyr1349), MKK3(Ser189), Myc(Ser373), Myc(Thr358), Myc(Thr58), P38 MAPK(Thr180), P38 MAPK(Tyr182), p44/42 MAP Kinase?Phospho-Thr202), p44/42 MAP Kinase(Tyr204), p53(Ser15), p53(Ser315), p53(Ser33), p53(Ser37), p53(Ser46), p53(Ser6), p53(Ser9), p53(Thr18), PKC delta(Ser645), PKC theta(Ser676), Pyk2(Tyr402), Raf1(Ser259), Raf1(Ser338), SAPK/JNK(Thr183), SAPK/JNK(Tyr185), SEK1/MKK4(Ser80), SEK1/MKK4(Thr261), Src(Tyr418), Src(Tyr529), Stathmin 1(Ser15), Stathmin 1(Ser24), Stathmin 1(Ser37), Tau(Ser214), Tau(Ser235), Tau(Ser262), Tau(Ser356), Tau(Ser396), Tau(Ser404), Tau(Ser422), Tau(Thr181), Tau(Thr205), Tau(Thr212), Tau(Thr231) |
| Target Source | Human; Mouse; Rat |
For Research Use Only.