| Overview | |
|---|---|
| Description | Creative Biolabs offers a wide range of reporter bioassays kits to characterize and develop novel monoclonal antibody (mAb) and cell-based therapeutics. The CD40 Bioassay Kit is a bioluminescent cell-based assay that measures potency and stability of ligands or agonist antibodies and other biologics that can bind and activate CD40. Based on the antibody properties to be tested, the CD40 Bioassay Kit can be conducted in single-cell systems to determine the agonist antibodies that can activate CD40. |
| Features | Convenience and minimizes variability; Easily applicable to high-throughput workflows. |
| Applications | To measure potency and stability of ligands or agonist antibodies and other biologics that can bind and activate CD40. |
| Application Notes | It is recommended to conduct a preliminary experiment before the formal experiment. |
| Molecular Mechanism of Action | In the absence of agonist antibody or CD40 ligand, the CD40 receptor is not activated and luminescence signal is low. The addition of CD40 ligand or antibody induces the CD40 pathway-activated luminescence, which can be detected in a dose-dependent manner by addition of Bio-Glo Reagent and quantitation with a luminometer. |
| Qualified With | Prequalified according to ICH guidelines |
| Assay Measures | Uses Bio-Glo Luciferase Assay System |
| Research Areas | Drug discovery; Function assay |
| Specification | |
|---|---|
| Size | 1EA |
| Components | 1 vial CD40 Effector Cells (0.5ml) 36ml RPMI 1640 Medium 4ml Fetal Bovine Serum 1 vial Bio-Glo Luciferase Assay Substrate (lyophilized) 10ml Bio-Glo Luciferase Assay Buffer |
| Target | CD40 |
| Species Reactivity | Human |
| Target/Description | CD40 is expressed on the surface of B cells, dendritic cells and monocytes and is a member of the tumor necrosis factor receptor superfamily. CD40 ligand (CD154) is the primary ligand for CD40 and is expressed by activated T cells, critical regulators of cellular and humoral immunity. Signaling via CD40 triggers activation of antigen-presenting cells (APC). |
| Alternative Names | Tumor necrosis factor receptor superfamily member 5 |
| UniProt ID | A0A0S2Z3C7 |
| Compatibility | Each kit contains sufficient reagents for 120 assays using the inner 60 wells of two 96-well plates which can do 4 antibody analysis (10 concentration dose*3 repeats). |
| Format | 96- and 384-well plate formats |
| Storage | Upon arrival, immediately transfer the cell vials to below -140°C (freezer or liquid nitrogen vapor phase) for long-term storage. Do not store cell vials submerged in liquid nitrogen. Do not store cell vials at -80°C as this will negatively affect cell viability and performance. Store Bio-Glo Luciferase Assay Buffer, Bio-Glo Luciferase Assay Substrate and Fetal Bovine Serum at -20°C. Avoid multiple freeze-thaw cycles of the serum. We recommend preparing Bio-Glo Reagent immediately before use. Do not store and use the reconstituted reagent. Store RPMI 1640 Medium at 4°C protected from fluorescent light. |
| Handling | Please place the product at proper storage condition once received. |
| Shipping | Components are shipped separately by dry ice and wet ice. |
For Research Use Only.
