Creative Biolabs has established a comprehensive and advanced drug discovery services platform. With years of experience in drug discovery, we can provide high-quality digital imaging services of functional analysis with numerous strategies.
Digital imaging is a technique to produce images, for example, an interior structure or a physical scene of a cell or tissue. This technique plays an essential role in the functional analysis of target identification and validation. Before observed by microscopes, target gene expression of the experimental group needs to be halt and compared the morphology changes (cell morphometry, sporulation) with wild types. Target location, function, and the virulence changes can be observed by various imaging techniques in this process. Gene expression usually deleted or disrupted by gene knockout, antisense technology or RNA interference (RNAi). Creative Biolabs offers diverse approaches for digital imaging services, including electron microscopy, fluorescent microscopy, confocal laser scanning microscopy, atomic force microscopy and radioautography.
Figure 1. Confocal laser scanning microscopy (CLSM) images of PGP 9.5-LIR (PGP 9.5) in taste buds of rat and mouse circumvallate papillae. (Ma et al. 2007)
Creative Biolabs has rich professional experience in high-quality digital imaging services with the advanced functional analysis platform, we can perform several strategies for digital imaging, including:
Electron microscopy (EM) is a technique by using a variety of methods to acquire high-resolution images, either biological or non-biological specimens. The scanning EM (SEM) and the transmission EM (TEM) are two types which have been widely used in biomedical studies to check the particular structure of organelles, cells, tissues, and macromolecular complexes.
Fluorescence microscopy is a technique designed with the purpose of only showing the specimen of interest in an otherwise black background. In confocal fluorescence microscopy, the actual 3D resolution is performed via energetically prohibiting any signal deriving from out-of-focus planes.
Confocal Laser Scanning Microscopy
Confocal laser scanning microscopy (CLSM) allows the reconstruction of three-dimensional structures from the procured images through gathering a series of images at different depths. Generally, CLSM is applied to the clinical diagnosis of eye diseases and is specifically used for qualitative analysis, imaging, and quantification of endothelial cells of the cornea.
Atomic force microscopy (AFM) is an essential method for researching biological samples, such as cells, tissues, and biomolecules, on account of its ability to image surfaces under liquids. AFM is the only technique available that directly provides structural, mechanical, and functional information at the high resolution of cells under physiological conditions.
By using radioautography (RAG), both the insoluble compounds tied to the macromolecules and the soluble compounds propagating in the cells and tissues can be demonstrated. Radioautography combined with electron microscopic techniques has been used to probe the distribution at the subcellular level.
Creative Biolabs provides other various drug discovery services. For more detailed information, please feel free to contact us or directly sent us an inquiry.
References
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