| Overview | |
|---|---|
| Description | Creative Biolabs offers a wide range of reporter bioassays kits to characterize and develop novel monoclonal antibody (mAb) and cell-based therapeutics. The GITR Bioassay Kits are bioluminescent cell-based assays used to measure the potency and stability of ligands or agonist antibodies that bind and activate GITR. The assay consists of a genetically engineered cell line, GITR Effector Cells, that expresses human GITR and a luciferase reporter driven by a response element that can respond to GITR ligand/agonist antibody stimulation. Based on the antibody properties to be tested, the GITR Bioassay Kit can be conducted in single-cell systems to determine the agonist antibodies that can activate GITR. |
| Features | Convenience and minimizes variability; Easily applicable to high-throughput workflows. |
| Applications | To measure the potency and stability of ligands or agonist antibodies that bind and activate GITR. |
| Application Notes | It is recommended to conduct a preliminary experiment before the formal experiment. |
| Molecular Mechanism of Action | The Bioassay Kit consists of one engineered cell line, GITR Effector Cells. In the absence of agonist antibody or GITR ligand, the GITR receptor is not activated and luminescence signal is low. The addition of agonist antibody or GITR ligand induces the GITR pathway-activated luminescence, which can be detected in a dose-dependent manner. |
| Qualified With | Prequalified according to ICH guidelines |
| Assay Measures | Uses Bio-Glo Luciferase Assay System |
| Research Areas | Drug discovery; Function assay |
| Specification | |
|---|---|
| Size | 1EA |
| Components | 1 vial x5 GITR Effector Cells (0.5ml x5) 36ml x5 RPMI 1640 Medium 4ml x5 Fetal Bovine Serum 1 vial x5 Bio-Glo Luciferase Assay Substrate (lyophilized) 10ml x5 Bio-Glo Luciferase Assay Buffer |
| Target | GITR |
| Species Reactivity | Human |
| Target/Description | GITR (CD357/TNFRSF18), a member of the tumor necrosis factor (TNF) receptor superfamily, is a costimulatory receptor widely expressed on most immune cells and further upregulated on activated T cells. When engaged with GITR ligand (GITRL) on the cell surface, GITR enhances subsequent T cell expansion and cytokine production including interleukin-2 (IL-2) and IL-9. |
| Alternative Names | TNFRSF18 |
| UniProt ID | Q9Y5U5 |
| Compatibility | Each kit contains sufficient reagents for 600 assays using the inner 60 wells of ten 96-well plates which can do 20 antibody analysis (10 concentration dose*3 repeats). |
| Format | 96- and 384-well plate formats |
| Storage | Upon arrival, immediately transfer the cell vials to below -140°C (freezer or liquid nitrogen vapor phase) for long-term storage. Do not store cell vials submerged in liquid nitrogen. Do not store cell vials at -80°C as this will negatively affect cell viability and performance. Store Bio-Glo Luciferase Assay Buffer, Bio-Glo Luciferase Assay Substrate and Fetal Bovine Serum at -20°C. Avoid multiple freeze-thaw cycles of the serum. We recommend preparing Bio-Glo Reagent immediately before use. Do not store and use the reconstituted reagent. Store RPMI 1640 Medium at 4°C protected from fluorescent light. |
| Handling | Please place the product at proper storage condition once received. |
| Shipping | Components are shipped separately by dry ice and wet ice. |
For Research Use Only.
