| Overview | |
|---|---|
| Description | Creative Biolabs offers a wide range of reporter bioassays kits to characterize and develop novel monoclonal antibody (mAb) and cell-based therapeutics. The ICOS Bioassay Kit is a bioluminescent cell-based assay used to measure the potency and stability of ligands or agonist antibodies that activate ICOS. Based on the antibody properties to be tested, the ICOS Bioassay Kit can be conducted with FcγRIIb aAPC/CHO-K1 Cells to determine if the agonist antibodies can activate ICOS in an FcγR-dependent manner. |
| Features | Convenience and minimizes variability; Easily applicable to high-throughput workflows. |
| Applications | To measure the potency and stability of ligands or agonist antibodies that activate ICOS. |
| Application Notes | It is recommended to conduct a preliminary experiment before the formal experiment. |
| Molecular Mechanism of Action | The ICOS Bioassay Kit for FcγRIIb-dependent agonist antibodies consists of two cell lines, ICOS Effector Cells and FcγRIIb aAPC/CHO-K1 Cells. In the absence of agonist antibody, ICOS is not activated and the luminescence signal is low. In the presence of FcγRIIb aAPC/CHO-K1 Cells, anti-ICOS antibody can be crosslinked, thereby inducing ICOS pathway-activating luminescence in a dose-dependent manner by adding Bio-Glo-NL™ Reagent and quantitating with a luminometer. |
| Qualified With | Prequalified according to ICH guidelines |
| Assay Measures | Uses Bio-Glo Luciferase Assay System |
| Research Areas | Drug discovery; Function assay |
| Specification | |
|---|---|
| Size | 1EA |
| Components | 1 vial x5 ICOS Effector Cells (0.5ml x5) 1 vial x5 FcγRIIb aAPC/CHO-K1 Cells (0.5ml x5) 72ml x5 RPMI 1640 Medium 4ml x5 Fetal Bovine Serum 1 vial x5 Bio-Glo Luciferase Assay Substrate (lyophilized) 10ml x5 Bio-Glo Luciferase Assay Buffer |
| Target | ICOS |
| Species Reactivity | Human |
| Target/Description | ICOS (CD278) binds to its ligand ICOSL (B7-H2, CD275), which is constitutively expressed on B cells, monocytes and dendritic cells, and can be induced on endothelial and epithelial cells during inflammation. ICOS co-stimulation induces the production of effector T cell cytokines such as interferon (IFN)-γ, interleukin (IL)-4 and IL-10. |
| Alternative Names | CD278 |
| UniProt ID | Q9Y6W8 |
| Compatibility | Each kit contains sufficient reagents for 600 assays using the inner 60 wells of ten 96-well plates which can do 20 antibody analysis (10 concentration dose*3 repeats). |
| Format | 96- and 384-well plate formats |
| Storage | Upon arrival, immediately transfer the cell vials to below -140°C (freezer or liquid nitrogen vapor phase) for long-term storage. Do not store cell vials submerged in liquid nitrogen. Do not store cell vials at -80°C as this will negatively affect cell viability and performance. Store Bio-Glo Luciferase Assay Buffer, Bio-Glo Luciferase Assay Substrate and Fetal Bovine Serum at -20°C. Avoid multiple freeze-thaw cycles of the serum. We recommend preparing Bio-Glo Reagent immediately before use. Do not store and use the reconstituted reagent. Store RPMI 1640 Medium at 4°C protected from fluorescent light. |
| Handling | Please place the product at proper storage condition once received. |
| Shipping | Components are shipped separately by dry ice and wet ice. |
For Research Use Only.
