| Overview | |
|---|---|
| Description | Creative Biolabs offers a wide range of reporter bioassays kits to characterize and develop novel monoclonal antibody (mAb) and cell-based therapeutics. The OX40 Bioassay Kit is a bioluminescent cell-based assay that measures the potency and stability of ligands or agonist antibodies that can bind and activate OX40. The assay consists of a genetically engineered cell line, OX40 Effector Cells, that expresses human OX40 and a luciferase reporter driven by a response element that can respond to OX40 ligand/agonist antibody stimulation. Based on the antibody properties to be tested, the OX40 Bioassay Kit can be conducted with FcγRIIb CHO-K1 Cells to determine the agonist antibodies that can activate OX40 in a FcγR-dependent manner. |
| Features | Convenience and minimizes variability; Easily applicable to high-throughput workflows. |
| Applications | To measure the potency and stability of ligands or agonist antibodies that can bind and activate OX40. |
| Application Notes | It is recommended to conduct a preliminary experiment before the formal experiment. |
| Molecular Mechanism of Action | The Bioassay Kit consists of two engineered cell lines, OX40 Effector Cells and and FcγRIIb CHO-K1 Cells. In the presence of FcγRIIb CHO-K1 Cells, the anti-OX40 antibody can be crosslinked, thereby inducing OX40 pathway-activated luminescence. |
| Qualified With | Prequalified according to ICH guidelines |
| Assay Measures | Uses Bio-Glo Luciferase Assay System |
| Research Areas | Drug discovery; Function assay |
| Specification | |
|---|---|
| Size | 1EA |
| Components | 1 vial OX40 Effector Cells (0.5ml) 1 vial FcgRIIb Cells (0.5ml) 72ml RPMI 1640 Medium 4ml Fetal Bovine Serum 1 vial Bio-Glo Luciferase Assay Substrate (lyophilized) 10ml Bio-Glo Luciferase Assay Buffer |
| Target | OX40 |
| Species Reactivity | Human |
| Target/Description | OX40 (CD134/TNFRSF4), a member of the tumor necrosis factor (TNF) receptor superfamily, is a costimulatory receptor expressed primarily on activated T cells, and on neutrophils and natural killer (NK) cells to a lesser extent. When present on the cell surface, OX40 interacts with OX40 ligand (OX40L) and induces subsequent cell proliferation, survival and production of cytokines, particularly in T cells. |
| Alternative Names | TNFRSF4 |
| UniProt ID | P43489 |
| Compatibility | Each kit contains sufficient reagents for 120 assays using the inner 60 wells of two 96-well plates which can do 4 antibody analysis (10 concentration dose*3 repeats). |
| Format | 96- and 384-well plate formats |
| Storage | Upon arrival, immediately transfer the cell vials to below -140°C (freezer or liquid nitrogen vapor phase) for long-term storage. Do not store cell vials submerged in liquid nitrogen. Do not store cell vials at -80°C as this will negatively affect cell viability and performance. Store Bio-Glo Luciferase Assay Buffer, Bio-Glo Luciferase Assay Substrate and Fetal Bovine Serum at -20°C. Avoid multiple freeze-thaw cycles of the serum. We recommend preparing Bio-Glo Reagent immediately before use. Do not store and use the reconstituted reagent. Store RPMI 1640 Medium at 4°C protected from fluorescent light. |
| Handling | Please place the product at proper storage condition once received. |
| Shipping | Components are shipped separately by dry ice and wet ice. |
For Research Use Only.
