| Overview | |
|---|---|
| Description | The kit is a complete and user-friendly system for measuring PXR activity in a cell-based luciferase reporter assay. It provides all the necessary reagents and protocols needed to accurately assess the transcriptional activity of the Human Pregnane X Receptor. |
| Features | Ready-used for receptor activity assay. |
| Applications | Kit is used to for compound screening in intact cells via luciferase reporter assay to quantify nuclear receptor activity. |
| Application Notes | Useful for screening of test samples to quantify any functional activity, either agonist or antagonist, that they may exert against human PXR. |
| Research Areas | Kinase Drug Discovery; Signal Pathway; |
| Specification | |
|---|---|
| Size | 96 tests |
| Components | The kit is supplied with an assay kit including PXR Reporter Cells, Cell Recovery Medium (CRM), Compound Screening Medium (CSM), 5a-Dihydro-11-keto Testosterone; (ref. agonist; in DMSO), Detection Substrate, Detection Buffer, and Assay Plate. |
| Target | Pregnane X Receptor |
| Species Reactivity | Human |
| Target/Description | Pregnane X Receptor (PXR) is a nuclear receptor that can be activated by a range of endogenous and xenobiotic compounds. It responds to specific ligands in a species-specific manner, including the antibiotic rifampicin and various plant metabolites such as hyperforin, guggulipid, colupulone, and isoflavones. |
| Format | Supplied with a luciferase reporter assay kit |
| Storage | Store at -80°C |
| Handling | Please store the kit according to the storage instructions in the kit's manual upon receipt. |
| Shipping | Dry ice |
| Regulatory Status | RUO |
For Research Use Only.
