Creative Biolabs is offering the most comprehensive services for antibody development projects. With strict regulation and effective execution, we are dedicated to providing the most valuable solutions to complete your projects.
HighlightsWith over a decade of experience in phage display technology, Creative Biolabs can provide a series of antibody or peptide libraries that are available for licensing or direct screening. These ready-to-use libraries are invaluable resources for isolating target-specific binders for various research, diagnostic or therapeutic applications.
HighlightsCreative Biolabs has established a broad range of platforms for developing novel antibodies or equivalents. These cutting-edge technologies enable our scientists to meet your demands from different aspects and tailor the most appropriate solution that contributes to the success of your projects.
HighlightsWith deep understanding in antibody-related realms and extensive project experience, Creative Biolabs offers a variety of references to help you learn more about our capacities and achievements, including infographic, flyer, case study, peer-reviewed publications, and all kinds of knowledge that can assist your projects. You are also welcome to contact us directly for more specific solutions.
HighlightsGet a real taste of Creative Biolabs, one of the most professional custom service providers in the world. We are committed to providing highly customized comprehensive solutions with the best quality to advance your projects.
HighlightsCreative Biolabs provides hybridoma cell line optimization services that aim to increase and stabilize the productivity of your original cell line for monoclonal antibody (mAb) production. Regarding your expectations, our innovative technology will be applied to your specific clone or pool, in order to maintain the high quality of your antibody product. Creative Biolabs is dedicated to assisting our clients in obtaining optimized hybridoma cell lines.
Our proprietary know-how is based on a succession of physical stimulation leading to improved cell metabolism and respiratory parameters required for achieving high and sustainable expression of the protein of interest. We develop customized strategies to generate the best hybridoma possible for each antibody produced. In order to provide maximum mAb yield during production, we will examine the viability and the clonality of provided hybridoma through our featured methods.
Each cell line will be evaluated in the following four steps:
1) Biological Testing
This step aims at evaluating cell lines for the presence of murine viruses or mycoplasma.
2) Recovery
Assess the viability of the hybridoma cells in the cryovial.
If the culture viability is low, we attempt to recover it and establish a healthy culture. If the culture viability is acceptable at thaw, recovery work is not required and we move directly to the next step. Once a healthy culture is established, the clonality of the cell line is determined.
3) Clonality Testing
In this step, the fraction of cells secreting mAb in a healthy culture is determined. MAb yield is improved when the clonality of the cell line is high. Failing to determine the clonality of the cell line prior to production may lead to unexpectedly low yields of monoclonal antibodies.
4) Subcloning
If the clonality of the culture is acceptable after step 3, the original culture is used for production work or cell banking work. If the clonality of the culture is unacceptable, Creative Biolabs subclones the original culture by selecting the best antibody-secreting clone. The subclone is used for production or cell banking.
A cell line is comprised of a large population of cells. Over time, mutations occur within the population. Some mutations give rise to virulent, non-antibody-producing cells which threaten the security of the cell line if they are permitted to grow. This condition is referred to as cell line drift. Therefore, continuous monitoring and cell line certification are important. At Creative Biolabs, large seed stocks can be maintained for each hybridoma cell line where they are closely monitored for hybridoma viability, the absence of microbes, antibody expression, and isotype uniformity.
After reviving the cell line, percent viability is determined with a trypan blue contrast assay. The cell line is then subcloned in order to assess monoclonality. Antibody activity is assessed with an immunoassay and the percent of specific antibody expression is calculated. The cell line is subcloned with the limiting dilution method to reinstate monoclonality preceding the development of a new cell bank. All cell lines are screened for mold, fungi microbial growth, mycoplasma, and viruses prior to the development of a certified hybridoma bank. Aliquots are stored to ensure the security of the cell line.
Besides, Creative Biolabs is capable of providing optimized mouse myeloma cell lines (NS0, SP2/0, etc) for cell fusion during the process of mAb preparation. Since each hybridoma development project is unique, successful projects will often require that immunization and screening protocols be tailored to the unique needs of the client. If you are interested in our hybridoma cell line optimization services, please feel free to contact us for more information.
All listed services and products are For Research Use Only. Do Not use in any diagnostic or therapeutic applications.
