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Fab-ScFv Fusion Protein Engineering Service

Based on the comprehensive antibody engineering platform established through years of experience, scientist from Creative Biolabs had successfully produced an important type Fab-scFv fusion protein using well established methods. These fusion proteins are multifunctional recombinant antibody derivatives, which utilize the natural in vivo hetero dimerization of the heavy chain and light chain of a Fab fragment, and thus form a scaffold incorporated with scFv binding domains.

Fig.1 Structure of monospecific IgG, Fab and scFv. Fig.1 The Fab-ScFv fusion.1

In the design of such a fusion protein, each chain can be extended preferably at the C-terminus with an additional scFv binder. The chains are co-produced in mammalian cells such as HEK293 and CHO, where the host-cell BiP chaperone drives the formation of the heavy chain-light chain heterodimers. These heterodimers are very stable (usually more stable than diabodies) with each of the binders retaining their specific affinities, higher affinity valence and higher activation of T cell proliferation and cytotoxicity in vivo.

Compared with other bispecific antibodies, such Fab-scFv fusion proteins have several advantages especially in therapeutic applications, their intermediate size (~100 kDa) between bispecific antibody fragments and IgG molecules translates into an ideal balance between tumor penetration and half-life; these molecules can be constructed using 3 distinct binders (tri-specific), 2 distinct binders with one in double copy (bivalent, bispecific), or three copies of the same binder (trivalent); they have similar bio-distribution patterns to IgG formats and even exhibit higher tumor accumulation rates than IgG formats.

Our Fab-scFv Fusion Protein Construction Services:

•  Design of Fab-scFv Fusion Protein
•  Expression and Purification
•  ELISA/ FACS/ Apoptosis Assay
•  Scale Up Production

Reference

  1. Pirkalkhoran, Sama, et al. "Bioengineering of antibody fragments: challenges and opportunities." Bioengineering 10.2 (2023): 122. Distributed under Open Access license CC BY 4.0. The image was modified by extracting and using only part of the original image.

All listed services and products are For Research Use Only. Do Not use in any diagnostic or therapeutic applications.

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