Close

HOC & SOC Dual Display based T4 Phage Library Construction Service

Background Service FAQ Resources

As a long-term expert in delivering a full range of comprehensive services based on phage display technology, Creative Biolabs keeps paving the way to revolutionary scientific progress. Currently, we proudly introduce this novel dual display service through bipartite fusions to HOC and SOC of bacteriophage T4 to our global clients.

Background

Phage display is an exponentially growing research area, which is being frequently applied in fields like immunology, cell biology, drug discovery and pharmacology. Molecules of interest, such as protein, peptide, and cDNA, can be displayed on the phage surface by genetically fusing to capsid proteins. In this way, a direct linkage between genotype and phenotype can be built up for further screening and analysis. Although filamentous phage display has always been a dominant choice and extensively utilized over decades, T4 phage display system recently emerges as a powerful approach for its unparalleled advantages.

Phage display platform based on bacteriophage T4 majorly employs two non-essential capsid proteins: HOC (39.1 kDa, 155 copies) and SOC (9 kDa, 810 copies). They are dispensable for capsid assembly and decorated into the phage particle during the last stage of maturation. Distinct from other structural capsids, HOC and SOC have no impact on the infectivity or viability of phage T4, hence confirmation changes of recombinant capsids due to foreign domain insertions will not interfere the phage assembly. On the other hand, because of the lytic life cycle of T4, progeny phages are assembled in the host cytoplasm and released by lysis of the cell envelope, avoiding the membrane extraction procedure which might cause host toxicity and protein misfolding.

T4 phage display platform using either HOC or SOC site has proved a great success in displaying large full-length proteins with considerate bioactivities, such as an antigen, antibody, receptor, enzyme, and so on. Here we present an exclusive HOC-SOC bipartite display system, which is able to simultaneously exhibit two different proteins on the T4 capsid surface. This novel platform has revealed tremendous potential for a variety of applications:

HOC & SOC Dual Display based T4 Phage Library Construction Service

With state-of-the-art technologies and seasoned team of experts, Creative Biolabs is professional in delivering high-quality service based on our innovative dual-display platform. Our scientists are pleased to promise precisely tailored and goal-oriented protocols according to assist our customers’ specific research objectives.

Head structure of T4 phage. (Creative Biolabs Original)Fig 1. Description of the T4 phage head structure.

Other optional T4 phage library construction service:

Reference
  1. Fokine, A., Islam, M. Z., Zhang, Z., Bowman, V. D., Rao, V. B. and Rossmann, M. G. (2011) 'Structure of the three N-terminal immunoglobulin domains of the highly immunogenic outer capsid protein from a T4-like bacteriophage', Journal of virology, 85(16), 8141-8148.

FAQ

  1. What is HOC & SOC dual display in T4 phage library construction, and how does it work?

    HOC & SOC dual display refers to the simultaneous use of both Highly Immunogenic Outer Capsid (HOC) and Smaller Outer Capsid (SOC) proteins in T4 phage display library construction. This method allows for the display of different peptides or proteins on both the HOC and SOC proteins, maximizing the diversity and functionality of the displayed library. The dual display system leverages the immunogenicity of HOC and the structural flexibility of SOC to create a versatile platform for screening a wide range of targets, enhancing the chances of identifying high-affinity binders or immunogenic epitopes.

  2. What are the primary advantages of using HOC & SOC dual display in T4 phage library construction?

    The primary advantages are increased versatility and functional diversity. By utilizing both HOC and SOC proteins, researchers can display multiple peptides on a single phage particle, enhancing the library's ability to target different biological pathways or immune responses. The dual display system also allows for the combination of high immunogenicity (from HOC) with structural accessibility (from SOC), making it a powerful tool for applications ranging from vaccine development to drug discovery.

  3. How is an HOC & SOC dual display-based T4 phage library constructed?

    The library construction involves inserting DNA sequences encoding different peptides or proteins into the genes encoding the HOC and SOC proteins of the T4 bacteriophage. These genetic modifications allow the phage to display peptides on both its highly immunogenic HOC protein and its smaller, more flexible SOC protein. The modified phage is then propagated in a bacterial host, producing a library of T4 phages that display diverse peptides on both capsid proteins, which can be screened for interactions with specific targets.

  4. What are the typical applications of HOC & SOC dual display-based T4 phage libraries?

    HOC & SOC dual display-based T4 phage libraries are used in drug discovery, vaccine development, and the study of protein-protein interactions. The dual display system's ability to present different peptides on both HOC and SOC proteins makes it particularly valuable for identifying ligands that bind to complex or multifaceted targets. Additionally, the immunogenic properties of HOC and the structural versatility of SOC make these libraries ideal for developing vaccines that require both strong immune responses and effective antigen presentation.

  5. How does the dual display of HOC and SOC enhance the screening process?

    The dual display of HOC and SOC provide a more comprehensive and flexible platform for peptide or protein interactions. The HOC protein's high immunogenicity ensures that the displayed peptides are readily recognized by the immune system, while the SOC protein's smaller size and accessibility allow for the display of peptides that require precise structural conformation. This combination increases the likelihood of identifying peptides that can bind to a wide range of targets with high affinity and specificity, making the screening process more effective and efficient.

  6. What is the significance of combining HOC and SOC displays in a single T4 phage library?

    Combining HOC and SOC displays in a single T4 phage library allows for the simultaneous exploration of both immune reactivity and structural interaction potential within the same library. This dual approach provides a more holistic view of peptide-target interactions, increasing the chances of identifying peptides that can function as effective vaccines, therapeutics, or diagnostic agents.

  7. How can HOC & SOC dual display-based T4 phage libraries be used in drug discovery?

    In drug discovery, HOC & SOC dual display-based T4 phage libraries are used to identify peptides or proteins that can bind to therapeutic targets with high specificity and affinity. The dual display system allows researchers to screen for ligands that interact with different aspects of a target's biology, whether through immune modulation (via HOC) or structural binding (via SOC). This versatility makes the dual display system particularly valuable for discovering novel drug candidates or optimizing existing therapeutics.

Resources

Use the resources in our library to help you understand your options and make critical decisions for your study.

VideosPodcastsInfographicFlyerCase studyArticles

All listed services and products are For Research Use Only. Do Not use in any diagnostic or therapeutic applications.

Online Inquiry
CONTACT US
USA:
Europe:
Germany:
Call us at:
USA:
UK:
Germany:
Fax:
Email:
Our customer service representatives are available 24 hours a day, 7 days a week. Contact Us
© 2025 Creative Biolabs. | Contact Us