Based on years of dedicated devotion and cutting-edge technology platforms, Creative Biolabs earns extensive expertise in antibody manufacturing and engineering by phage display technique. Scientists from Creative Biolabs now proudly introduce professional service of T4 phage display library construction using smaller outer capsid (SOC) to our worldwide clients.
T4 phage display system emerges as a promising technique for various applications, such as antibody development, vaccine design, diagnostic assays and drug discovery. In contrast to the widely used filamentous phage display platform, this method takes advantage of the unique dispensable capsid protein and lytic life cycle of bacteriophage T4, and sets up a completely distinct display system. Adoption of T4 phage display can offer a powerful solution when conventional M13 phage display meets obstacles.
Smaller outer capsid (SOC) is one of the two non-essential proteins on the icosahedral head of bacteriophage T4. SOC (9 kDa) has a tadpole-shaped molecule structure, which enables binding between gp23 hexons (main capsid protein) and up to 810 copies per phage. In situ, SOC is presented as trimers at the trigonal position of the gp23 lattice, while as monomers at the sites nearest to the vertices. As a dispensable capsid, SOC is incorporated into the mature virions after completion of the assembly. Lack of SOC will have no impact on the infectivity or viability of T4. However, SOC binding is suggested to provide great stability to phage T4 under hostile environments like extreme pH (pH 11), high temperature (60 °C) and denaturing reagents.
SOC’s small size (9 kDa), high density (810 copies) and other characteristics create a rich binding platform, making a suitable vehicle for phage display. Moreover, the lytic lifecycle of phage T4 eliminates the membrane extraction, which avoids the inserted size limitation, host toxicity, and protein misfolding during the secreting procedure. Both N- and C-termini are available for foreign insertions, and C-terminal SOC display is most commonly used.
So far, SOC fusions have successfully displayed various macromolecule complex with high density, which is evidenced in multiple research fields. Notably, T4 phage display on SOC reveals the great potential for vaccine design and personalized drug development. Aided by our cutting-edge technology platforms and experienced scientists, Creative Biolabs offers our global customers this exclusive opportunity to obtain comprehensive service of T4 phage display library construction on SOC. We are capable of conducting tailored protocols to generate customized libraries based on specific project objectives.