Drug-induced Liver Injury (DILI) Evaluation by 3D Microtissue Model

Drug-induced liver injury (DILI) continues to be a major concern for the pharmaceutical business as well as a primary factor in drug development attrition, withdrawal post-marketing, and cautionary/restrictive labeling patient care. Creative Biolabs launches various 3D microtissue models to help you with DILI evaluation and related research.

DILI Evaluation By 3D Microtissue Model

Three-dimensional (3D) microtissues or spherical cell aggregates are one of the ideal models for 3D culture and drug screening at the cellular level due to their good reproducibility and similarity to in vivo environments. Human 3D liver microtissues/spheroids are effective in vitro models for DILI research.

Advantages

Recapitulation of liver physiology: 3D microtissue models are engineered to mimic the structure and function of liver tissue, including the presence of hepatocytes, stellate cells, endothelial cells, and Kupffer cells, creating a microenvironment that closely resembles the in vivo liver.
Improved cell-cell interactions: Unlike 2D cell cultures, which lack proper cell-cell interactions, 3D microtissue models enable the formation of cell-cell junctions and complex cellular interactions that are crucial for maintaining liver function.
Enhanced drug metabolism and toxicity prediction: The presence of multiple cell types in 3D microtissue models allows for improved drug metabolism and clearance predictions. Hepatocytes in 3D models display enhanced metabolic activity and maintain drug-metabolizing enzyme expression closer to their in vivo counterparts, resulting in more accurate toxicity predictions.
Increased sensitivity to drug-induced toxicity: 3D microtissue models exhibit higher sensitivity to drug-induced liver injury compared to traditional 2D cultures. This is attributed to the improved cell viability, differentiation, and function that these models offer, allowing for the detection of subtle signs of hepatotoxicity.

Applications in Drug Development

Early toxicity screening	Mechanistic understanding	Personalized medicine
3D microtissue models can be utilized in the early stages of drug development to screen for potential liver toxicity, enabling the identification of hepatotoxic drug candidates before further development.	These models offer a platform for studying the underlying mechanisms of drug-induced liver injury, helping researchers gain insights into the cellular and molecular events involved in toxicity.	3D microtissue models can be derived from patient-specific induced pluripotent stem cells (iPSCs), which allows for the development of personalized models for studying individual variations in drug metabolism and toxicity response.

The use of 3D microtissue models provides a valuable approach for evaluating DILI. Creative Biolabs offers various 3D microtissue models for drug development, toxicity screening, etc. Please contact us to discuss your research requirements.

Research Model

3D Biology Based Biomarker Discovery

3D Biology Based Drug Discovery and Development

3D Biology Based Toxicity Evaluation

Supporting Assays

Drug-induced Liver Injury (DILI) Evaluation by 3D Microtissue Model

DILI Evaluation By 3D Microtissue Model

Applications in Drug Development

Related Sections: