What is a stable overexpressing cell line?

A stable overexpressing cell line is a cell line in which a specific gene is introduced and stably expressed for an extended period of time. The overexpressed gene is integrated into the cell's genome, allowing for continuous expression in subsequent generations of cells.

How long can the stable overexpressing cell line be maintained?

These cell lines are designed to maintain stable expression of the overexpressed gene for many passages, ensuring consistency in experimental results. However, it's important to regularly check for the gene expression levels over time, as silencing can occasionally occur.

Can I obtain a stable overexpressing cell line that expresses multiple genes?

Yes, it is possible to create a stable cell line that expresses multiple genes through co-transfection or using multi-gene vectors. However, the expression levels of each gene may need to be optimized.

GRAMD1C Overexpression Stable Human Huh7 Cell Line (CAT#: DBFF11-HX390)

Datasheet

Overview

GRAMD1C Overexpression Human Huh7 Cell Line is a stable cell line. It carries a puromycin resistance gene for selection and an EGFP fluorescent marker. This model is ideal for studies involving GRAMD1C expression, pathway analysis, and drug screening.

Quantity:

* For Research Use Only. Not For Clinical Use.

Specifications

Species

Human

Cell Line

Huh7

Cell Type

Gene overexpressing cell lines

Cell Status

Normal

Format

Cryopreserved

Product details

Quality Control

Negative for bacteria, fungi and mycoplasma.

Viability Examination After Recovery

Pass

Sequencing Detection

Pass

PCR Detection

Pass

Product properties

Storage

Liquid nitrogen

Shipping

Dry ice

Advantages

Advantages

Mature gene editing technology
Stable product supply
Fast delivery time

Gene information

Gene

GRAMD1C

Research Area

Gene function, signaling pathway, disease mechanism and others.

FAQs

What is a stable overexpressing cell line?
A stable overexpressing cell line is a cell line in which a specific gene is introduced and stably expressed for an extended period of time. The overexpressed gene is integrated into the cell's genome, allowing for continuous expression in subsequent generations of cells.
How long can the stable overexpressing cell line be maintained?
These cell lines are designed to maintain stable expression of the overexpressed gene for many passages, ensuring consistency in experimental results. However, it's important to regularly check for the gene expression levels over time, as silencing can occasionally occur.
Can I obtain a stable overexpressing cell line that expresses multiple genes?
Yes, it is possible to create a stable cell line that expresses multiple genes through co-transfection or using multi-gene vectors. However, the expression levels of each gene may need to be optimized.

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For Research Use Only | Not For Clinical Use