How should these cells be thawed to ensure optimal viability?

Place the cryovial immediately into a 37°C water bath and agitate gently until only a small ice crystal remains (typically within 1 minute). Dilute rapidly with pre-warmed complete growth medium and centrifuge at low speed to remove the cryoprotectant.

What is the typical passage recommendation for these primary cells?

These primary cells maintain robust growth and functional characteristics for 3-5 passages under optimal culture conditions. Using early-passage cells ensures high biological relevance in in vitro assays.

How does Creative Biolabs ensure lot-to-lot consistency and cell purity?

Each lot of HLA-typed Human Lung Primary Fibroblasts undergoes strict quality control testing, including sterility screening (free of bacteria, fungi, and mycoplasma), cell viability verification, and specific biomarker characterization.

HLA-typed Human Lung Primary Fibroblasts (CAT#: DBFF-0926-HX94)

Datasheet

Overview

HLA-typed Human Lung Primary Fibroblasts supplied by Creative Biolabs are meticulously isolated from healthy human lung tissue. These primary cells maintain native physiological features and surface biomarkers, making them an indispensable in vitro tool for extracellular matrix synthesis and wound healing research.

Quantity:

* For Research Use Only. Not For Clinical Use.

Specifications

Species

Human

Organ/Tissue

Lung

Source

Healthy human donors

Cell Type

HLA-typed human primary cells

Cell Status

Normal

Format

Cryopreserved

Product details

Quality Control

Negative for mycoplasma, bacteria, fungi, HIV, HBV, and HCV.

Applications

HLA matching and mismatch studies, immune response research, cell and gene therapy research, drug immunogenicity studies, and disease modeling.

Product properties

Storage

Liquid nitrogen

Shipping

Dry ice

FAQs

How should these cells be thawed to ensure optimal viability?
Place the cryovial immediately into a 37°C water bath and agitate gently until only a small ice crystal remains (typically within 1 minute). Dilute rapidly with pre-warmed complete growth medium and centrifuge at low speed to remove the cryoprotectant.
What is the typical passage recommendation for these primary cells?
These primary cells maintain robust growth and functional characteristics for 3-5 passages under optimal culture conditions. Using early-passage cells ensures high biological relevance in in vitro assays.
How does Creative Biolabs ensure lot-to-lot consistency and cell purity?
Each lot of HLA-typed Human Lung Primary Fibroblasts undergoes strict quality control testing, including sterility screening (free of bacteria, fungi, and mycoplasma), cell viability verification, and specific biomarker characterization.

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For Research Use Only | Not For Clinical Use