3D Biology Based Immune Checkpoint Assays

Creative Biolabs has assembled experts in the application of 3D biological models to provide effective 3D biology-based immune checkpoint assays.

Strategies for 3D Biology Based Immune Checkpoint Assays

A tissue engineering approach to building the 3D ex vivo model platform.

The target biological material for the construction of the 3D ex vivo model platform to be assayed needs to be first collected and isolated, e.g. from in vivo, ex vivo, stem cells, or tumor cells. After selecting the scaffold material for model growth support, the model is constructed and guided with bioactive factors in the normal or tumor state 3D model.

Labeling of immune checkpoints.

Specific antibodies are used to label immune checkpoints, including but not limited to bioassays and biochemical assays for PD-1, CTLA-4, CD47, OX40, and LAG3, as well as blockade bioassays for CD278. Notably, the constructed tumorigenic 3D model can naturally carry a state of simulated immune checkpoint blockade.

Immunostaining and analysis.

The labeled antibodies are combined with immune cells or tissues in the sample and the markers are visualized by methods such as fluorescent staining and histochemistry. Images are acquired and quantitative analysis is performed.

Fig 1. Ex vivo culture from fresh or slow-frozen matching tumor samples.^1,2

Immune Checkpoint Assay Items Based on 3D Biology

• Evaluate the level of immune cell infiltration and immunological checkpoint protein expression.
• Detection of mRNA transcript levels of immune checkpoints in 3D models.
• Identify the function based on cell proliferation tests, ELISA, etc.
• Statistics and analysis of the detected immune checkpoint expression and function data, including the comparison of differences between groups, correlation analysis, etc.

Advantages

• On the localization and quantification, it may offer more exact information.
• Evaluate the efficacy and predict the immune responsiveness of agents.
• Contribute to a fuller comprehension of the immunosuppressive approach taken by tumors in evading immune attack.
• Enable assessment of subjects' sensitivity to immune checkpoint inhibitors in individualized medicine and guide the selection of treatment regimens.

Fig 2. Schematic representation of the project workflow.^1,3

Creative Biolabs has focused on expanding the application of 3D biological modeling for many years and involved technologies, such as multilayer stacking of cells and construction of internal vascular networks, to provide emerging 3D biology-based immune checkpoint assay services. For further information on how we may assist with your desired assay, please kindly contact us.

Related Sections:

• 3D Biology Based Multi-Omics Analysis Services
• 3D Biology Based Immune Response Analysis Services
• 3D Biology Based Target Analysis and Validation Services