Inhalation Toxicity Evaluation by Human 3D Airway Model

Creative Biolabs specializes in providing inhalation toxicity evaluation services by human 3D airway models and has in-depth experience in testing a wide range of agents including drugs, vaccines, hormones, and agrochemicals.

Human 3D Airway Model Description

Tissue source	Human trachea/bronchial epithelium from healthy or diseased individuals.
Cell composition	Human tracheal/bronchial epithelial cells grow and differentiate into pseudostriated epithelium on inserts at the fluid/air interface.
Structure	3-4 cell layers, highly physiological 3D mucociliary tissue system.
Characteristics	Summarize the morphological features of the source epithelium, immunolabeling shows cytokeratin positivity.
Functions	Infectious, mucociliary, and virulent reactions.
Quality control	No bacteria, fungi, yeasts, viruses, or mycoplasma.

Detailed Characterization and Analysis of a Three-Dimensional Airway Model. Fig. 1 Characterization of a 3D airway model.¹

Human 3D Airway Model Applications

Examination of epithelial function and mechanisms, including proliferative changes, epithelial permeability, and tissue repair.
Investigation of airway-related diseases, such as lung injury, airway infections, inflammation, and smoking.
Evaluation of inhalation toxicity.
Compound efficacy screening and expansion for high throughput assays.

Inhalation Toxicity Evaluation Procedures by Human 3D Airway Model

Tissue equilibrated overnight and transferred to fresh testing medium environment.
Preparation of test solutions
- Positive control: sterile diH2O or corn oil
- Negative control: formaldehyde
- Test conditions: various specified concentrations of sterile diH2O or corn oil + tested materials
Tissues exposed to the designated dose of nebulized/vaporized tested material (n=3 tissues/condition) with vapor cap to prevent material evaporation.
Transfer tissues to the recovery medium after exposure.
Determine relative tissue viability (MTT method) and histologic assays to comprehensively evaluate inhalation toxicity.
Other endpoints: measurements of released cytokines and trans-epithelial electrical resistance, etc. to assess the effect of the tested material on the barrier function of the model.

An illustration of assessing the inhalation toxicity of tire wear particles via a 3D airway model. Fig. 2 An example of evaluating the inhalation toxicity of tire wear particles by a 3D airway model.¹

Advantages of the Evaluation Strategy

Realistic simulation	Quantitative assessment
The 3D airway model simulates the human airway system, including the structures of the nose, mouth, throat, trachea, and lungs, allowing for a more accurate prediction of how the tested material transfers and acts after entering the airway.	3D airway modeling allows quantitative assessment of key parameters such as inhalation, deposition, and absorption of the tested material, which can serve as references for safety programming and risk management, comparing with relevant safety standards and guideline values.
Reproducibility	Efficient detection
The model eliminates the individual differences and other confounding factors of traditional airway models, facilitating more consistent and comparable inhalation toxicity evaluation results.	Using 3D airway models significantly reduces the modeling turnaround time and allows rapid screening of large numbers of candidates for potential toxicity, assisting in determining the toxicity risk of the tested material at an earlier stage.

At Creative Biolabs, our most specialized team in 3D airway modeling and inhalation toxicology provide inhalation toxicity evaluation services by human 3D airway model to effectively explore the safety of your chemicals and drugs. Please do not hesitate to contact us to discuss your project.

Reference

Jiang, Yingying et al. "Human airway organoids as 3D in vitro models for a toxicity assessment of emerging inhaled pollutants: Tire wear particles." Frontiers in bioengineering and biotechnology. 10 (2023): 1105710. Distributed under Open Access license CC BY 4.0, without modification.

Research Model

3D Biology Based Biomarker Discovery

3D Biology Based Drug Discovery and Development

3D Biology Based Toxicity Evaluation

Supporting Assays

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