What is the main application of Luciferase Reporter Cell Lines?

Luciferase Reporter Cell Lines are used primarily in gene expression studies, signal transduction assays, drug screening, toxicity testing, and promoter activity assays. They provide a sensitive and non-invasive method to monitor gene expression and cellular activity in real-time.

How do Luciferase Reporter Cell Lines work?

The cell line is engineered to express luciferase in response to a specific promoter or regulatory element. When the target gene or pathway is activated, the luciferase enzyme is produced, and upon the addition of luciferin substrate, light is emitted. The intensity of the light correlates with the level of gene activation or pathway activity, allowing for quantification.

Luciferase Reporter Human TPC-1 Cell Line (CAT#: DBFF11-HX472)

Datasheet

Overview

The Luciferase Reporter Human TPC-1 Cell Line is a stable cell line engineered using a lentiviral vector to express the luciferase gene. Puromycin was used for the selection of successfully transduced cells. This reporter cell line enables sensitive luminescence-based detection of promoter activity or pathway activation. Each vial contains 1.0 × 10⁶ cryopreserved, quality-controlled cells.

Quantity:

* For Research Use Only. Not For Clinical Use.

Specifications

Species

Human

Organ/Tissue

Thyroid

Cell Type

Luciferase-expressing cell lines

Cell Status

Normal

Format

Cryopreserved

Product details

Quality Control

Negative for bacteria, fungi and mycoplasma.

Applications

Used for monitoring gene expression, analyzing signal transduction pathways, screening drugs, studying promoter activity, and evaluating cellular responses.

Product properties

Storage

Liquid nitrogen

Shipping

Dry ice

FAQs

What is the main application of Luciferase Reporter Cell Lines?
Luciferase Reporter Cell Lines are used primarily in gene expression studies, signal transduction assays, drug screening, toxicity testing, and promoter activity assays. They provide a sensitive and non-invasive method to monitor gene expression and cellular activity in real-time.
How do Luciferase Reporter Cell Lines work?
The cell line is engineered to express luciferase in response to a specific promoter or regulatory element. When the target gene or pathway is activated, the luciferase enzyme is produced, and upon the addition of luciferin substrate, light is emitted. The intensity of the light correlates with the level of gene activation or pathway activity, allowing for quantification.

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For Research Use Only | Not For Clinical Use