This product is a Ramos (RA 1) B cell engineering tool based on the Gold nanoparticles (N-acetyl-D-glucosamine,sialic acid/Wheat germagglutinin) delivery system and CRISPR-based (SHS-317-SpCas9-NLS-Cas9-SCR7 pyrazine ) genome editor.
| Host Cell | Ramos (RA 1) |
| Target | ALPP |
| Insert Clone | E6 |
| Safe Harbor Sites (SHS) | SHS-317 |
| gRNA | SpCas9 |
| Nuclease | NLS-Cas9 |
| Enhancer | SCR7 pyrazine |
| Targeting Strategy | pH responsive |
| Insertion sites | Large DNA sequences |
| Vehicles | Gold nanoparticles |
| Ligand/Receptor | N-acetyl-D-glucosamine, sialic acid/Wheat germ agglutinin |
| Active Agents | Thymoquinone |
| Particle Size | 1-100 |
| Charge (mv) | 0 to -30 |
| Total Atom Count | ~20,000,000 |
| Surface Atoms (%) | ~4 |
| Specific Surface Area (m2/g) | ~8 |
| Vector Type | MMLV |
| Vector Name | BCpCDH5 |
| Targeting Diseases | Breast cancer; Lung cancer; Colorectal cancer |
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