This product is a B cell engineering plasmid designed by genomic integration of B cell receptors (GUS-hPGK-Tat/SPI-CD79α-CD8-Myc) and CRISPR-Cas (Cas12b (C2c1)-dsDNA-RuvC, HNH-5' PFS: non-C; 3' PFS:NAN/NNA-Stat5b) systems.
| Clone | 33 V11 |
| Host | Mouse |
| Targeting Cell Type | Farage-Luc2 |
| Reporter gene | GFP |
| Promoter | UBC |
| Signal peptides | Tat/SPI |
| Signaling domains | CD79α |
| TM domains | CD8 |
| Detection tags | Myc |
| Linkers | Flexible Linker |
| Co-Receptors | CD22 |
| Vector Type | RABV |
| Vector Name | BGpCDH8 |
| Class | 1 multi-Cas |
| Type | I |
| Subtype | A |
| Effector | Cas12b (C2c1) |
| DNA Type | dsDNA |
| Nuclease domains | RuvC, HNH |
| TracrRNA requirement | No |
| PAM/PFS | 5' PFS: non-C; 3' PFS:NAN/NNA |
| Insertion | Stat5b |
| Construction | GFP-UBC-CD79α-Cas12b (C2c1)-dsDNA-Stat5b |
| Format | Liquid/lyophilized |
| Shipping | It is usually shipped overnight at room temperature in small cardboard boxes. If hot temperatures are expected during transit, they will be shipped in polystyrene containers with gel ice packs. |
| Handling Advice | No special measures are necessary. Keep away from food, drink and animal feedingstuffs. |
| Storage | Plasmid (lyophilized), short-time storage at Room Temperature, recommended store at -20°C or -80°C ; Plasmid (Liquid), blue ice store at 4°C. |
Certificate of Analysis Lookup
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