This product is a B cell engineering plasmid designed by genomic integration of B cell receptors (YFP-EFS-Tat/SPI-CD79β-CD28-His) and CRISPR-Cas (Cas13a (C2c2)-ssRNA-RuvC, Nuc-5' AT-rich PAM-ROSA26) systems. This product can precisely introduce cassettes of several kb size into the genome of various B cells to enhance surface expression and antigen recognition of a synthetic BCRs.
| Clone | KN332 |
| Host | Human |
| Targeting Cell Type | Ramos (RA 1) |
| Reporter gene | YFP |
| Promoter | EFS |
| Signal peptides | Tat/SPI |
| Signaling domains | CD79β |
| TM domains | CD28 |
| Detection tags | His |
| Linkers | Glycine-Serine linker |
| Co-Receptors | CD72 |
| Vector Type | Baculovirus-AAV |
| Vector Name | BGpCDH4 |
| Class | 1 multi-Cas |
| Type | I |
| Subtype | A, B |
| Effector | Cas13a (C2c2) |
| DNA Type | ssRNA |
| Nuclease domains | RuvC, Nuc |
| TracrRNA requirement | No |
| PAM/PFS | 5' AT-rich PAM |
| Insertion | ROSA26 |
| Construction | YFP-EFS-CD79β-Cas13a (C2c2)-ssRNA-ROSA26 |
| Target | TNF |
| Alternative Names | Tumor Necrosis Factor; Tumor Necrosis Factor Ligand Superfamily Member 2; Cachectin; TNF-Alpha; TNFSF2; TNF-A; TNFA; Tumor Necrosis Factor (TNF Superfamily; Member 2); Tumor Necrosis Factor Ligand 1F |
| Gene ID | 7124(Human); 21926(Mouse) |
| UniProt ID | P01375(Human); P06804(Mouse) |
| Format | Liquid/lyophilized |
| Shipping | It is usually shipped overnight at room temperature in small cardboard boxes. If hot temperatures are expected during transit, they will be shipped in polystyrene containers with gel ice packs. |
| Handling Advice | No special measures are necessary. Keep away from food, drink and animal feedingstuffs. |
| Storage | Plasmid (lyophilized), short-time storage at Room Temperature, recommended store at -20°C or -80°C ; Plasmid (Liquid), blue ice store at 4°C. |
Certificate of Analysis Lookup
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.