Recombinant Anti-Ad fiber knob x Anti-EPCAM Bispecific Antibody (Diabody) is designed to be expressed as two polypeptide chains respectively. The two chains are cross-over pairing to form a dimer. It contains a His-tag for affinity purification. This BsAb can retarget of adenoviral vectors to tumour cells. It can be used in cancer gene therapy to transfer a therapeutic gene into tumour cells aiming at selective and efficient cell killing.