Introduction of CLIC4
Chloride intracellular channel 4 (CLIC4) protein is encoded by the CLIC4 gene, which is a member of the p64 family and found in many tissues. The CLIC4 gene exhibits an intracellular vesicular pattern in PANC-1 cells (pancreatic cancer cells). Gene ontology annotations associated with this gene include chloride channel activity and voltage-gated chloride channel activity. An important paralog of this gene is CLIC5. Chloride channels are a diverse group of proteins which regulate basic cellular processes. Chloride channels stabilize cell membrane potential, transepithelial transport, maintain intracellular pH and regulate cell volume. Related pathways of CLIC4 include hepatic ABC transporters and activation of cAMP-dependent PKA.
|Basic Information of CLIC4|
|Protein Name||Chloride intracellular channel protein 4|
|Aliases||Intracellular chloride ion channel protein p64H1|
|Organism||Homo sapiens (Human)|
Function of CLIC4 Membrane Protein
High cell proliferation rate, migration, and invasiveness are specific properties of tumorigenesis, which involves chloride channels. CLIC4 is present in the mitochondrial membrane, cytoplasm, and nucleus as a soluble protein. CLIC4 is expressed in the mitochondria and regulates intracellular pH and cell volume. Under normal physiological conditions, CLIC4 is localized in the nucleus through the nuclear localization signal (NLS). The functional activity of CLIC4 is determined by its redox state: oxidant conditions enhance its membrane binding capacity and channel activity. CLIC4 is also involved in multiple cellular processes such as apoptosis, inflammation and endothelial tubulogenesis. However, CLIC4 is almost absent in normal stromal tissues and highly expressed in cancer tissues and tumor-related stromal fibroblasts. CLIC4 may be a potential target for certain cancers.
Fig.1 Structure of the CLIC4 protein.
Application of CLIC4 Membrane Protein in Literature
The article reveals that siRNA-induced down-regulation of CLIC4 could enhance proliferation, while in turn promoting apoptosis of mouse liver cancer Hca-F and Hca-P cells.
The article indicates that CLIC4 and Ihh can be used as biomarkers for the diagnostic of pancreatic ductal adenocarcinoma, including progression, metastasis, and invasiveness.
Authors in this group demonstrated that CLIC4 is not essential for collaterogenesis but is required for perinatal maturation of nascent collaterals.
This article focuses on the possible role of CLIC4 in cell adhesion and β1 integrin trafficking. CLIC4 was defined as a modulator of Rab35 activity and serum- and LPA-dependent integrin trafficking.
This article reports that CLIC4 expression is regulated particularly in fetal liver, thymus, kidney, brain, and heart, as well as developing brown adipose tissue and stratifying epidermis during the second and third trimesters.
CLIC4 Preparation Options
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