Anti-Membrane Protein Antibody Discovery Service by Humanized Transgenic Mice

Creative Biolabs now introduces multiple brand-new humanized transgenic mice to discover fully human anti-membrane protein antibodies in a most straightforward and convenient manner. Incorporated with this leading-edge technology as well as skillful technique support, we provide one-stop human antibody development service for both research and industrial customers.

Introduction

The discovery and development of antibodies against membrane proteins are critical for therapeutic and diagnostic applications. These proteins, which reside on the cell surface, play crucial roles in cell signaling, communication, and transportation, making them prime targets for drug development, particularly in cancer and autoimmune diseases. However, the generation of high-affinity and specific antibodies against such proteins is notoriously challenging due to their hydrophobic nature and conformational complexity. In recent years, the use of humanized transgenic mice has revolutionized the field of monoclonal antibody discovery. These genetically-engineered mice are designed to contain human antibody genes, enabling them to produce fully human antibodies. This technology circumvents some of the limitations associated with traditional antibody generation methods, such as reduced immunogenicity and enhanced efficacy in human therapeutic applications.

Methodology

The process begins with the immunization of humanized transgenic mice with carefully prepared antigens corresponding to the target membrane proteins. Following immunization, B cells from these mice are harvested and screened for the production of antibodies with desired specificity and affinity. Advanced techniques such as hybridoma technology or phage display libraries are then utilized to identify and clone the most promising antibody candidates. Creative Biolabs has launched an innovative transgenic mouse strain producing 100% human antibodies (including Fc regions). Apart from this, high immune performance can also be guaranteed. We have successfully generated 3 humanized transgenic mice strains capable of producing human antibodies with VH-VL, heavy chains only and constant VL. Immunization tests reveal this mouse model can react potently to many membrane proteins, especially GPCRs. Incorporated with multiple antibody discovery methodologies like hybridoma, phage display, B cell sorting, humanized mouse can be a powerful toolbox to streamline the antibody drug discovery pipeline.

Features of Transgenic Mice from Creative Biolabs

Multiple strains and powerful antibody discovery approaches: offer flexible choices to meet customers' demands.
100% human sequence: avoid risky and tedious in vitro humanization process.
Versatile immunogen formats: with years of experience in this regard, Creative Biolabs has built a comprehensive membrane protein immunogen preparation platform.
Potent immune response: Our humanized transgenic mice have been proven to mount vigorous immune responses against many molecules, particularly membrane proteins.

Creative Biolabs has been dedicated to leveraging our cutting-edge technologies and expertise to facilitate all kinds of custom projects. We are more than happy to provide professional assistance with anti-membrane protein antibody projects to worldwide clients. Please feel free to contact us for more information.

All listed services and products are For Research Use Only. Do Not use in any diagnostic or therapeutic applications.

Online Inquiry

	USA: Europe: Germany:
	Call us at: USA: UK: Germany:
	Fax:
	Email: