Anti-Membrane Protein Antibody Discovery Using Hybridoma

Hybridoma technology has long been the classic approach and milestone for the generation of monoclonal antibodies. With years of experiences in membrane protein production and antibody discovery, Creative Biolabs now provides anti-membrane protein antibody discovery service using hybridoma to our worldwide clients.

Membrane proteins, consisting of 20~30% total encoding genes, make up over 50% of current therapeutic targets. Nowadays, anti-membrane protein antibody becomes more and more popular in clinical due to their high specificity, high affinity, and low cytotoxicity compared with small molecules. As a well-established methodology, hybridoma technology is widely used to produce anti-membrane protein antibodies for a variety of applications.

Advantages of Anti-Membrane Protein Antibody Discovery Using Hybridoma

Anti-Membrane Protein Antibody Discovery Using Hybridoma Fig 1 Hybridoma selection. (Ruigrok V, 2011)

To provide flexible options, we have following species from rodents to develop anti-membrane protein antibodies based on our well-served murine hybridoma production and rat hybridoma generation services:

Rat is a popular rodent used in the laboratory except for mouse. Undoubtedly, rats are the ideal source for anti-membrane protein antibody discovery for greater lymphocyte yields than mice. Rat monoclonal antibodies offer several other advantages over mice, such as the ability to induce the immune response to mouse antigens. Besides, rat antibodies pair better than that of mouse in sandwich assays. Moreover, the rat antibodies do not have the cross-reaction in immune-detection of antigens out of a mouse background. This service package includes one-stop service from immunogen preparation, immunization, hybridoma selection and anti-membrane protein antibody production.

Mouse is a well-known mammalian model organism in biology. Mouse hybridoma technology is the most widely and successfully used approach for the production of mouse monoclonal antibodies. Compared with other familiar experimental animals, mice take advantages of their small size, lower feeding cost, ease of maintenance and handling and strong ability to reproduce. Moreover, mice share similar antibody isotypes with human. Creative Biolabs has a special humanized transgenic mice platform, on which the genetically modified mice are capable of raising high-affinity human antibodies after immunization, thus eliminating long humanization and affinity maturation process. We tailor service and many pre-made monoclonal antibodies in stock.

As a dedicated service provider, Creative Biolabs is also professional in tailoring challenging membrane proteins in functional immunogen formats. With the help of our membrane protein preparation as antigen for antibody discovery service, multiple formats of membrane proteins including liposomes, polymers, nanodiscs, designed peptides, DNA immunization, whole cell presentation, etc. can be utilized for immunization and antibody selection. With our Ph.D. level science team and years of experience, plenty of GPCR immunogens, ion channel immunogens, and transporter immunogens have been successfully obtained. We are more than happy to facilitate your projects and push the progress towards pre-clinical trials. Please feel free to contact us for more details.


  1. Ruigrok V J, Levisson M, Eppink M H, et al. (2011). Alternative affinity tools: more attractive than antibodies? Biochemical Journal. 436(1), 1-13.

All listed customized services & products are for research use only, not intended for pharmaceutical, diagnostic, therapeutic or any in vivo human use.

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