Development of Electrochemical Biosensor

Electrochemical biosensors overcome the limitations of miniaturization and cost presented in optical biosensors and opens the possibility to apply these devices to point-of-care (POC). Up to now, electrochemical biosensors have been used in the detection of various targets such as cancer biomarkers, chemotherapeutics or antibodies. Armed with avaliable practical strategies and successful completion of projects, Creative Biolabs is competent to develop electrochemical biosensors capable of rapidly and reliably detect the presence of interested targets for worldwide clients to support their research use.

Introduction

Biosensors arise as tools capable of producing continuous signals that are proportional to the number of binding molecules that react on the surface of a sensor. Generally, biosensors comprise three parts:

1. The sensitive elements which are biologically derived material such as ssDNA.
2. The transducer or detector element that transforms the detected signal into a readable, quantified output.
3. The signal processor that displays the transformed signal in a user-friendly way.

Working Principle

The design of the electrochemical DNA biosensor is based on the hybridization dynamics of nucleic acid. When the target DNA contains a sequence that matches that of the immobilized oligonucleotide-probe DNA, a hybrid duplex DNA is formed at the electrode surface. Such a hybridization event is usually detected through the changed current signals of certain electroactive indicators, connected to the use of enzyme labels or redox labels, or from other hybridization-induced changes in electrochemical parameters.

Fig.1 General design of electrochemical DNA biosensor. (Liu, 2012)

Important Considerations in Our Services

In order to guarantee good sensitivity of the biosensor, the stability of probes plays a very important role in the overall preparation of an electrochemical DNA biosensor. Besides, the design of the DNA electrochemical probe is the key factor in the fabrication of the electrochemical DNA biosensor. Creative Biolabs has accumulated a wealth of experience in years of project design work and optimized our biosensor development strategies. Many important factors, such as the ones listed below, should be taken into consideration.

Services

There are different methods to monitor DNA hybridization, including amperometry, voltammetry, potentiometry, and conductimetry. With the development of the integration of magnetic particles within biosensing devices, magnetic particles have been widely used in electrochemical biosensor. Pre-enrichment and purification are thus achieved simply by applying an external magnet. Creative Biolabs provides one-stop electrochemical biosensors for our honor clients. Some essential steps are listed as follows.

• Aptamer selection/modification
• Electrochemical biosensor design
• Fluorescence anisotropic analysis
• Electrode preparation and biosensor attachment
• Sensor behavior analysis

Creative Biolabs has addressed feasible methods to increase the sensitivity of electrochemical signals by implementing labeling techniques applicable to a variety of electroactive nanotracers. The electrochemical biosensors have been shown to be suitable for high-performance analysis in diverse field applications. If you are interested in our electrochemical biosensor development services, please feel free to contact us for more information.

Reference

1. Liu, A.; et al. Development of electrochemical DNA biosensors. TrAC Trends in Analytical Chemistry. 2012, 37: 101-111.

For Research Use Only.