Recent years, electrochemical approaches have received particular attention due to their rapid detection and great sensitivity for the evaluation of DNA-hazard compounds interaction mechanisms. Creative Biolabs has developed different types of bioanalytical method using nucleic acids probes to detect DNA damage. With rich experience and advanced platform, Creative Biolabs firmly grasps the current directions and strategies in the development and applications of electrochemical DNA sensors for the detection of DNA damage. We provide customized electrochemical DNA biosensors development services for DNA damage detection.
DNA is a very important biomolecule that has an essential role in the determination of hereditary characteristics. Many compounds bind and interact with DNA causing changes to the structure of DNA and the base sequences, leading to perturbations in DNA replication or cancer. Therefore, it is very important to illustrate the factors that determine affinity and selectivity in binding molecules to DNA, identify these chemical species and ascertain their potency so that human exposure to them can be minimized.
Oxidative DNA damage caused by oxygen-free radicals leads to various modifications in DNA, such as base-free sites and oxidized bases. The major product of DNA oxidative damage is 8-oxo-7,8-dihydroguanine (8-oxoGua). 8-oxoGua, the deoxynucleoside form of 8-oxodGuo, has been the subject of intensive investigation and has become widely accepted as a biomarker of oxidative DNA damage and cellular oxidative stress. At present, HPLC with electrochemical detection (HPLC-ECD) and HPLC with tandem mass spectrometry (HPLC-MS/MS) are the most commonly used technique to assess urinary 8-oxodGuo.
The oxidation of the other DNA bases is much more difficult due to their high oxidation potentials. Oxidative damage to DNA by free radicals and exposure to ionizing radiation generates several products within the double helix besides 8-oxoGua, such as 2,8-dihydroxyadenine, 5-formyluracil, and 5-hydroxycytosine.
The development of electrochemical DNA-biosensors has opened a wide perspective using particularly sensitive and selective methods for the detection of specific interactions. Compared with other transducers, electrochemical ones received particular interest due to a rapid detection and great sensitivity. Combining the characteristics of DNA probes with the capacity of direct and label-free electrochemical detection represents an attractive solution in the detection of DNA base damage.
Among various electrochemical transducers, carbon electrodes exhibit some unique properties and the wide electrochemical potential window in the positive direction allows sensitive electrochemical detection of oxidative damage caused to DNA by monitoring the appearance of oxidation peaks of DNA bases. To design efficient DNA-electrochemical biosensors, Creative Biolabs is fully armed with knowledge concerning the structure and the electrochemical characteristics of DNA molecules. We are able to provide customized DNA damage detection services according to your project needs.
Fig.1 Schematic representation of DNA electrochemical biosensor design and operation. (Chiorcea-Paquim, 2021)
Electrochemical DNA-biosensors have proved to be excellent tools for investigating the effect of various endogenous and exogenous sources of hazard to genomic material, allowing quick and low cost determination of DNA damage. If you are interested in our services, please feel free to contact us for more information.
Reference
For Research Use Only.
