Creative Biolabs is an undisputed leading provider of antibody development and generation services. Now, we provide in vitro diagnostic (IVD) antibody development services targeting various biomarkers of a wide variety of diseases. Especially, Creative Biolabs offers high-quality antibody development services for diagnostic applications targeting DDX46 as a potential biomarker of lymph cancer.
Probable ATP-dependent RNA helicase DDX46 (DDX46)
Probable ATP-dependent RNA helicase DDX46 is an enzyme that in humans is encoded by the DDX46 gene. This gene encodes a member of the DEAD box protein family. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. The protein encoded by this gene is a component of the 17S U2 snRNP complex; it plays an important role in pre-mRNA splicing.
Fig.1 DDX46 increases the retention of antiviral mRNA in the nucleus and decreases the expression of the protein encoded. (Zheng, Q., 2017)
DDX46 Marker of Lymph Cancer
It has been well noted that the processes involved in vertebrate hematopoiesis during development consist of two evolutionarily conserved steps: primitive hematopoiesis followed by definitive blood formation. In the definitive hematopoiesis stage, all blood lineages arise from self-renewing hematopoietic stem cells (HSCs), and hematopoiesis derived from HSCs persists for the lifetime of vertebrates. Balanced and precisely controlled processes between self-renewal and differentiation of hematopoietic stem cells (HSCs) into all blood lineages are critical for vertebrate definitive hematopoiesis. In recent years, zebrafish has been used as an excellent vertebrate system for studying hematopoiesis during development.
DDX46 is required for the development of digestive organs and the brain, possibly by regulating pre-mRNA splicing. Zebrafish DDX46 is expressed in HSCs of the caudal hematopoietic tissue (CHT). The number of HSCs expressing the molecular markers cmyb or T-cell acute lymphocytic leukemia 1 (tal1) is markedly reduced in DDX46 mutants. However, massive cell death of HSCs is not detected, and proliferation of HSCs is normal in the CHT of the mutants at 48 h post-fertilization. It is found that myelopoiesis occurs, but erythropoiesis and lymphopoiesis are suppressed, in DDX46 mutants.
Antibodies are core elements for antibody-based immunoassays for detecting and quantifying antigens of interest in all kinds of samples such as serum, urine, and tissue preparations. IVD antibodies are extensively used for disease screening, prognosis, and therapeutic monitoring. With our versatile IVD platform, Creative Biolabs is proud to develop novel DDX46-specific antibody from scratch to commercial IVD kit (we can also start with provided antibody candidates).
Creative Biolabs has successfully completed a number of IVD antibody generation and development projects for clients across the globe. If you are interested in our services, please contact us to discuss your project needs.
Reference
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