Multiplex Biomarker Analysis

Creative Biolabs is professional in biomarker assay development, validation, and profiling with multiplex biomarker panels, using immunoanalytical and enzymatic platforms, or LC-MS/MS, when appropriate. We have substantial experience with custom multiplex designs and can expertly help you design a custom biomarker assay to suit your needs.

Promising Multiplex Immunoassays

The simultaneous measurement of different targets from a single sample is an emerging area for achieving efficient and high-throughput detection. Multiplex immunoassays confer obvious advantages over widely used singleplex assays such as greater output per sample volume ratios, increased efficiency at a reduced expense, and higher throughput predicting more resolute. Contemporary multiplex immunoassay systems may be divided into two formats: planar assays and microbead-based suspension assays.

Fig.1 Multiplex formats in common use include planar-based assays or suspension-based assays. (Tighe, et al., 2015)

• For planar format, high-affinity capture ligands are immobilized discretely on a solid phase. The immobilized ligands are then exposed to treatment with the sample and probed with detection antibodies labeled with a reporter system.
• For suspension format, high-affinity capture ligands are immobilized discretely on fluorescently activated plastic microbeads and mixed with the sample in the liquid phase. Subsequent addition of detection antibodies labeled with a reporter dye enables high-resolution analysis of specific fluorescent signals via flow cytometric methods.

On-Demand Assay Design and Development

Our scientists will help to design and qualify study-specific biomarker assays. Although the ELISA is regarded as a singleplex assay format, the method can also be made suitable for multiplex protein analysis. Monoclonal, polyclonal, or recombinant antibodies with a defined specificity, sensitivity, and stability can be combined in one test to allow the binding of multiple proteins. We can transfer and qualify assays or develop them for you. Fast and accurate testing services are available once the assay is developed.

Fig.2 Labeled- and label-free strategies for electrochemical sensing of protein biomarkers. (Van Gool, et al., 2020)

As a nimble biomarker CRO, we offer singleplex or multiplex assays on innovative platforms or as commercially available ELISA assays evaluated using a traditional 96-well plate format. All these assays undergo a fit-for-purpose biomarker validation, including assessing bioanalytical parameters such as accuracy, precision, and sensitivity. Once the biomarker assay is well characterized and robust, it can be relied upon to analyze preclinical samples. Specialized in biomarker assay development, we maintain high-quality standards in each step. Some of our most popular biomarker assay services include assay development, method validation, and cytokine testing.

Our biomarker platform offers several complementary solutions to fit every need you may have in terms of protein quantitation and profiling, as well as phosphorylation or glycosylation profiling, and mRNA/miRNA profiling. If you are interested in our multiple biomarker analysis services, please feel free to contact us for more information.

References

1. Tighe, P.J.; et al. ELISA in the multiplex era: potentials and pitfalls. PROTEOMICS-Clinical Applications. 2015, 9(3-4): 406-422.
2. Van Gool, A.; et al. Analytical techniques for multiplex analysis of protein biomarkers. Expert Review of Proteomics. 2020, 17(4): 257-273.

For Research Use Only.