Single B Cell Screening (Sorting) Overview

With the deepening of research, monoclonal antibodies play more and more roles in the field of biomedicine by virtue of their unique advantages. For example, it can be used in the development of test medical diagnostic reagents or as a ligand in affinity chromatography to participate in the purification of proteins. It can also be used in tumor-directed therapy and radioimmunoassay for immunologic phenomena. The development of monoclonal antibodies, on the other hand, can be prepared by virtue of the single B cell sorting technique, in addition to utilizing traditional hybridoma technology and display systems. Antibodies prepared by this method have the advantages of good genetic diversity, high efficiency, and full human origin. Based on our rich field experience and advanced B-cell sorting platform, Creative Biolabs provides comprehensive services to support the development and application of single B cell screening.

Single B Cell Screening

Currently, methods commonly used for single B-cell screening include fluorescence activated cell sorting (FACS), laser capture microdissection (LCM), microengraving, and droplet microfluidics. Each of these methods has its own advantages and disadvantages, and the appropriate method needs to be selected according to the specific needs of the study.

The FACS assay is designed based on antigen- and antibody-specific reactions. It utilizes fluorescein-labeled antibodies to B cell surface molecules as well as labeled specific antigens to screen for antigen-specific B cells by multicolor flow analysis and sorting. FACS allows for the sorting of individual antigen-specific B cells into single wells of a cell culture plate and the direct amplification and determination of antibody genes. This technique is one of the more widely used methods. In practice, FACS enables rapid and accurate separation of large numbers of B cells and also allows simultaneous analysis of multiple parameters.

LCM is capable of handling a wide variety of tissue samples and allows for the visualization of tissue or cellular samples. It is also capable of handling in vivo samples, which meets a wider range of research needs. Microengraving is a method of recognizing and cloning antigen-specific B cells based on soft lithography microarray chips. It generates antibodies by stimulating polyclonal B cells and distributing them one by one into the chip wells for culture. Then the antibody in the modified chip wells is transferred to the corresponding protein chip, which reacts with the fluorescent antibody after reacting with the target antigen. Finally, according to the results of fluorescent antibody staining, the B cells secreting specific antibodies are transferred to cell culture dishes by microscopic manipulation for subsequent cloning operations or antibody gene amplification.

Antibodies obtained from single B cell sorting have many advantages, such as full human origin, high self-specificity, and homogeneity. These features make them unique and promising for the treatment of pathogenic microbial infections, tumors, autoimmune diseases, and organ transplantation.

In the treatment of pathogenic microbial infections, antibodies obtained using other production techniques can only study a limited variety of B cells. However, single B cell antibody technology can help researchers quickly isolate memory B cells and prepare single antibodies, even months or years after a patient has been infected or immunized. At the same time, people can understand and elucidate the mechanism of the human immune system more deeply from the genetic level. Currently, single B-cell sorting technology has been used to prepare highly specific human antibodies against pathogenic microbial infections, such as HIV antibodies and tetanus antibodies.

In the field of autoimmune disease detection and treatment, studies have shown that ELISA technology based on single B cell screening produces more effective diagnostic results. The most prominent application of single B cell antibody technology is in the treatment and study of autoimmune diseases and the human immune system. It is capable of isolating B cells from any period of time in the human body, which makes it possible to study in detail the function and mechanism of the human immune system at all stages.

Despite the wide application prospects of single B cells, their development is still constrained by some technical constraints. For example, high-throughput PCR antibody gene amplification technology is not perfect enough, certain target antigens are difficult to prepare, and suitable human donors are in high demand. Therefore, in-depth research in the field of single B cell screening is still needed to further promote its development.

More Details on Single B Cell Screening (Sorting)

Introduction to FACS Technology for Single Cell Isolation
Introduction to LCM Technology for Single Cell Isolation
Introduction to Microengraving Technology for Single Cell Isolation
Introduction to Droplet Microfluidics for Single Cell Isolation

Creative Biolabs has a wealth of knowledge and experience in single B cell screening. We would be happy to share with you our knowledge and experience.

All listed services and products are For Research Use Only. Do Not use in any diagnostic or therapeutic applications.