Creative Biolabs is offering the most comprehensive services for antibody development projects. With strict regulation and effective execution, we are dedicated to providing the most valuable solutions to complete your projects.
HighlightsWith over a decade of experience in phage display technology, Creative Biolabs can provide a series of antibody or peptide libraries that are available for licensing or direct screening. These ready-to-use libraries are invaluable resources for isolating target-specific binders for various research, diagnostic or therapeutic applications.
HighlightsCreative Biolabs has established a broad range of platforms for developing novel antibodies or equivalents. These cutting-edge technologies enable our scientists to meet your demands from different aspects and tailor the most appropriate solution that contributes to the success of your projects.
HighlightsWith deep understanding in antibody-related realms and extensive project experience, Creative Biolabs offers a variety of references to help you learn more about our capacities and achievements, including infographic, flyer, case study, peer-reviewed publications, and all kinds of knowledge that can assist your projects. You are also welcome to contact us directly for more specific solutions.
HighlightsGet a real taste of Creative Biolabs, one of the most professional custom service providers in the world. We are committed to providing highly customized comprehensive solutions with the best quality to advance your projects.
HighlightsMonoclonal antibodies have advantages such as high targeting and high specificity, which make them unique in the field of disease treatment. In recent years, single B cell sorting technology has been active in the field of antibody production. Antibodies obtained based on single B cell sorting technology have the advantages of good genetic diversity and high efficiency. And microengraving is a commonly used technique for single B cell isolation. Single B-cell sorting technology based on microengraving has unique advantages in the development of monoclonal antibodies. Based on our rich field experience and B-cell sorting platform, Creative Biolabs provides comprehensive services to support the development and application of microengraving technology for single cell isolation.
Single B-cell antibody preparation technology is an in vitro cloning and expression of single antigen-specific B-cell antibody gene technology. This technique can preserve the natural pairing of the variable regions of the light and heavy chains and has the advantages of good genetic diversity, high efficiency, full human origin, and a small number of cells required. Therefore, it is now widely used in the development of monoclonal antibodies in the field of disease treatment. Nowadays, common methods used for single B cell isolation include fluorescence activated cell sorting, microengraving, laser-capture microdissection, and droplet microfluidics. In practice, these methods have their own advantages and shortcomings, so they need to be carefully screened when choosing.
Microengraving technology is based on the method of recognizing and cloning antigen-specific B cells on soft lithography microarray chips. Antibodies are produced by stimulating polyclonal B cells and distributing them one by one into the chip wells for culture. The antibody in the wells of this chip is then transferred to the corresponding protein chip and reacted with fluorescent antibody by reacting with the target antigen. Finally, according to the fluorescent antibody staining results, the B cells secreting the specific antibody are transferred to a cell culture dish for subsequent cloning operations or antibody gene amplification by microscopic manipulation. Immunospot microarray assay on a chip is an extension of the microengraving method. It replaces the antibody transfer process with anti-IgG antibodies encapsulated on the surface of the chip and enables the study of antibodies specific for a number of different target antigens and the isolation of a variety of antigen-specific B cells on the same chip. Compared with the original micro-engraving method, immunospot array assays on a chip simplifies the experimental process and is more efficient, making it a very promising method for sorting B cells. These two methods are characterized by high throughput, high specificity, and high sensitivity. Their disadvantages are the high price of chip array construction and the complexity of antibody encapsulation on the chip. Therefore, there will be higher requirements in terms of experimental cost and technical threshold.
Microengraving techniques have now been successfully used to obtain novel monoclonal antibodies against many different types of antigens. These antibodies include tetramers of major histocompatibility proteins, peptide-KLH couplers, and complex mixtures of cellular antigens. In the production of these antibodies, the number of potential antibodies identified in each primary screen far exceeded the number of cell lines that, for practical reasons, could be retrieved by manual micromanipulation and yielded new monoclonal antibodies against the target targets.
Creative Biolabs has a wealth of knowledge and experience in single B cell screening. We would be happy to share with you our knowledge and experience related to microengraving technology for single cell isolation.
All listed services and products are For Research Use Only. Do Not use in any diagnostic or therapeutic applications.
