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HighlightsSodium-dependent phosphate transport protein 4, also known as solute carrier family 17 member 3 (SLC17A3), is a protein that in humans is encoded by the SLC17A3 gene. SLC17A3 is expressed in the kidney and localized to the apical side of renal tubules. SLC17A3 is a voltage-driven transporter that excretes intracellular urate and organic anions from the blood into renal tubule cells. Two transcript variants encoding different isoforms have been found for SLC17A3 gene. The longer isoform is a plasma membrane protein with transporter activity, while the shorter isoform is localized to the endoplasmic reticulum.
| Basic Information of SLC17A3 | |
| Protein Name | Sodium-dependent phosphate transport protein 4 |
| Gene Name | SLC17A3 |
| Aliases | Na(+)/PI cotransporter 4, NPT4, Sodium/phosphate cotransporter 4, Solute carrier family 17 member 3 |
| Organism | Homo sapiens (Human) |
| UniProt ID | O00476 |
| Transmembrane Times | 8 |
| Length (aa) | 420 |
| Sequence | MATKTELSPTARESKNAQDMQVDETLIPRKVPSLCSARYGIALVLHFCNFTTIAQNVIMNITMVAMVNSTSPQSQLNDSSEVLPVDSFGGLSKAPKSLPAKSSILGGQFAIWEKWGPPQERSRLCSIALSGMLLGCFTAILIGGFISETLGWPFVFYIFGGVGCVCCLLWFVVIYDDPVSYPWISTSEKEYIISSLKQQVGSSKQPLPIKAMLRSLPIWSICLGCFSHQWLVSTMVVYIPTYISSVYHVNIRDNGLLSALPFIVAWVIGMVGGYLADFLLTKKFRLITVRKIATILGSLPSSALIVSLPYLNSGYITATALLTLSCGLSTLCQSGIYINVLDIAPRYSSFLMGASRGFSSIAPVIVPTVSGFLLSQDPEFGWRNVFFLLFAVNLLGLLFYLIFGEADVQEWAKERKLTRL |
NPT4 (SLC17A3), classified as a type I sodium-phosphate cotransporter, serves as a voltage-driven organic anion efflux transporter at the apical side of renal proximal tubules. There are two splice variants of SLC17A3 mRNA. One variant, NPT4_L, contains an open reading frame encoding a putative 498-amino-acid protein, and the other, hNPT4_S, encodes a 420-amino-acid protein. SLC17A3 mediates the efflux of drugs such as para-aminohippurate (PAH) and diuretics and that containing endogenous substrates such as urate in a voltage-sensitive manner. It is documented that sialin uses proton co-transport to drive transmembrane movement of acidic sugars, while urate transport by SLC17A1 and SLC17A3, glutamate transport by the VGLUTs, and ATP transport by VNUT. The characterized transporters all exhibit dependence on Cl- with the exception of sialin.
Fig.1 The uric acid transportasome. (Merriman, 2015)
In this article, authors demonstrated that the diuretics entered proximal tubular cells via basolateral OAT1 and/or OAT3 and might then interfere with the NPT4-mediated apical urate efflux in the renal proximal tubule, which might lead to the induction of hyperuricemia.
The authors revealed that C allele of rs548987of SLC17A3 showed significant association with small vessel disease subtype of ischemic stroke.
The authors proposed that NPT4 was an important transepithelial proximal tubular transporter that transported diuretic drugs and operated functionally with basolateral organic anion transporters 1/3 (OAT1/OAT3).
The authors found that the K(m) of OTxA for the efflux transporter hNPT4 was much higher than those for the uptake transporters hOAT1 and hOAT3, favoring the accumulation of OTxA in the tubular cell and leading to nephrotoxicity.
This article revealed the genomic structure of NPT4 and analyzed the gene as a candidate for glycogen storage disease type Ic (GSD Ic).
To obtain the soluble and functional target protein, the versatile Magic™ membrane protein production platform in Creative Biolabs enables many flexible options, from which you can always find a better match for your particular project. Aided by our versatile Magic™ anti-membrane protein antibody discovery platform, we also provide customized anti-SLC17A3 antibody development services.
Creative Biolabs' skillful scientists are glad to leverage our expertise and advanced technologies to help you with the member protein research. If you are interested, please feel free to contact us for more details.
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