Yeast Display Platform

Background Platform Advantages

With extensive experience in this field, Creative Biolabs has developed and commercialized a wide range of innovative services based on our Ultraff™ yeast display platform which benefit from the eukaryotic expression, ease of genetic manipulation, high protein expression, and convenient isolation of binders through FACS.

What Is Yeast Display?

Yeast display, or yeast surface display, is an advanced protein engineering technique that involves expressing recombinant proteins on the surface of yeast cells. By fusing a protein of interest to the C- or N-terminus of these anchors, up to 100,000 copies of the fusion protein can be displayed on Saccharomyces cerevisiae. This method is particularly powerful for isolating and engineering antibodies, offering a unique combination of eukaryotic expression and high-throughput screening capabilities. Since its introduction in 2000, yeast surface display has been extensively utilized to enhance protein properties, including affinity, specificity, expression, stability, and catalytic activity.

Versatile Applications of Yeast Display

The applications of yeast display have expanded to the display of proteins and enzymes for various biotechnology and biomedical applications such as:

Fig. 1 A schematic diagram for the yeast display system. (Creative Biolabs Original) Fig. 1 Yeast display of protein of interest.

Protein or Antibody Engineering
Enzyme Engineering and Optimization
Protein Epitope Mapping
Identification of Protein-Protein Interactions

Immobilized Proteins and Enzymes
Vaccine Development
Immune Biocatalysis and Biosensors

Advantages of Yeast Display over Phage Display

Yeast Display Platform in Creative Biolabs

Creative Biolabs builds the Ultraff™ Yeast Display Platform to create highly diverse and efficient libraries, ideal for high-affinity antibody discovery and affinity maturation. Our unique yeast transformation protocol achieves up to 2×10⁸ transformants per transformation.

Comprehensive Services via Ultraff™ Yeast Display

Creative Biolabs offers a wide range of innovative integrated services based on Ultraff™ yeast display platform, including but not limited to:

Yeast Display Library Screening
Protein Optimization and Engineering
Peptide Discovery

Advantages of Ultraff™ Yeast Display Platform

High Throughput Screening

Yeast display allows for the rapid screening of large libraries, enabling the identification of high-affinity binders or functional proteins from vast combinatorial libraries.

Eukaryotic Expression System

Yeast provides a eukaryotic environment that can perform proper folding and post-translational modifications, including glycosylation, disulfide bond formation, and appropriate protein folding.

Quantitative Analysis

The technology allows for quantitative binding measurements using flow cytometry that enables selective isolation of populations with varying affinity levels, facilitating precise quantitative analysis.

Ease of Genetic Manipulation

Yeast is a well-characterized and easily manipulated organism. Genetic modifications, such as introducing mutations or creating libraries, can be done relatively easily and efficiently.

Mutagenesis and Evolution

Yeast display is highly amenable to directed evolution approaches, where iterative cycles of mutation and selection can be used to evolve proteins with enhanced properties, such as increased stability, binding affinity, or enzymatic activity.

Compatibility with Other Systems

Yeast display can be integrated with other technologies, such as phage display and next-generation sequencing.

To explore the full potential of yeast display technology with Creative Biolabs' comprehensive services and expert support, please send us a note or call us directly at +1-631-416-1478.

All listed services and products are For Research Use Only. Do Not use in any diagnostic or therapeutic applications.

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