Although phage display has been widely used in the industry of protein engineering, including antibody discovery and optimization, it has been reported that phage display is highly biased in its ability to display antibodies derived from different human antibody germline variable region genes, or even antibody clones that differ with only a single point mutation. In contrast, Creative Biolabs’ yeast display library shows no such bias. Creative Biolabs offers the most comprehensive portfolio of yeast display library construction related services, including yeast display antibody library construction services, yeast display non-antibody protein library construction, yeast display peptide library construction, and yeast display cDNA library construction services.
Yeast Display Library Construction
Yeast surface display (YSD) has been regarded as an increasingly powerful tool for protein engineering and library screening applications. It can be used to screen large expressed protein libraries for proteins or protein fragments with specific binding properties. Yeast display offers selected advantages in relation to other display technologies, most notably eukaryotic expression of the heterologous target protein, making it an outstanding tool for display and engineering of many proteins that are difficult to produce in other display formats.
Beyond standard PCR-based methods for generating diversity, several other methods have been used to create diverse libraries that can be screened by the surface display. As one example, scFvs against a number of different small molecule and protein targets were isolated from a 109-member nonimmune scFv library comprised of assembled heavy and light chain genes mimicking the natural germline diversity found in human B-lymphocytes. If instead, it is desired to bias a library toward a specific antigen, pseudo-immune and immune libraries of scFv can be created.
Yeast display libraries are constructed by cloning the genes coding for antibody fragments into vectors that can be transformed into the yeast cells. Generally, the methods of yeast display library construction can be divided into four categories: 1) Generation of frameshift variants of yeast display vector; 2) Library construction; 3) Transformation of library into yeast; 4) Test library induction.
Fig.1. Creation of novel proteins/peptides with improved or developed function by yeast display library (Kuroda and Ueda, 2013).
Yeast Display Library Construction in Creative Biolabs
As a world leader in the industry of antibody discovery, Creative Biolabs has extensive experience in yeast display library construction using our exclusive Ultraff™ Yeast Display Technology. Library diversity can also be enhanced by utilizing the ability of haploid yeast to efficiently mate leading to the formation of a diploid organism. Our yeast display library construction services including:
By utilizing the advanced technology, our scientists are willing to construct various yeast display libraries for our worldwide clients.
If you are interested in learning more about Creative Biolabs’ yeast display library constrcution services, please contact us for more details.
All listed services and products are For Research Use Only. Do Not use in any diagnostic or therapeutic applications.