Gastrointestinal Toxicity Evaluation by Intestinal Organoid Model

Creative Biolabs specializes in intestinal organoid culture technology and applies it to in vitro intestinal modeling to provide reliable gastrointestinal toxicity evaluation services by intestinal organoid model.

Intestinal Organoid Culture System Introduction

Starting Cells	Intestinal adult stem cells	Human pluripotent stem cells
Culture	Starting cells are cultured in a matrix gel that simulates the microenvironment of intestinal stem cell growth. This culture condition also contains signals that simulate the organization and polarization of epithelial cells in vivo.
Organoid formation	The model contains cells undergoing intestinal stemness during the earlier stages of formation, followed by further differentiation into mature intestinal cells.	The model contains simultaneously the intestinal stem cell population, terminally differentiated intestinal epithelial cells and associated mesenchymal cells.
	The intestinal cell population automatically arranges into the epithelial layer in a crypt-villus structure under appropriate conditions.
Organoid cell composition	Key intestinal cell types such as intestinal epithelial cells, enteroendocrine cells, and intestinal stem cells.
Organoid maintenance	Can be passed on for long terms or stored frozen.
Genetic characterization	Consistent with donor-specific genetic background. Can be gene-edited in vitro.

Mechanical cleavage and enlargement of human intestinal organoids. Fig 1. Mechanical splitting and expansion of human intestinal organoids.¹

Gastrointestinal Toxicity Evaluation by Intestinal Organoid Model

Symptoms of gastrointestinal toxicity	Evaluation techniques
Frequent villus blunting and atrophy Mucosal damage and ulceration Capillary dilation and hemorrhage Barrier dysfunction Immune disorders and inflammation	Histological analysis and immunohistochemistry Sequencing Mass spectrometry Tissue viability assays

Advantages of Toxicity Evaluation Approach Involving Intestinal Organoid Model

Highly reproducible physiologic structure of the intestinal epithelium and the pathologic structure in response to toxic agent stimulation.
Easy and flexible modeling with removing the possibility of introducing confounding variables in the in vivo experiments to improve the stability of gastrointestinal toxicity assays.
Actively dividing intestinal stem cells ensure the easy long-term maintenance and expansion of intestinal organoids.
Significant in vitro advantages as an alternative to applying animal models for toxicity testing.

Other Gastrointestinal Toxicity Evaluation Services

Gastrointestinal Toxicity Evaluation by 3D Cell Model

Intestinal organoid models are capable of reproducing the intestinal lumen and surrounding intestinal epithelial structures. At Creative Biolabs, we assist in the design and implementation of solutions to meet your needs by incorporating intestinal organoid models into the gastrointestinal toxicity evaluation workflow. Please contact us for further details.

Reference

Pleguezuelos-Manzano, Cayetano et al. "Establishment and Culture of Human Intestinal Organoids Derived from Adult Stem Cells." Current protocols in immunology. 130,1 (2020): e106. Distribution under Open Access license CC BY 4.0, without modification.

Research Model

3D Biology Based Biomarker Discovery

3D Biology Based Drug Discovery and Development

3D Biology Based Toxicity Evaluation

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