HLA-typed Human Biliary Primary Epithelial Cells supplied by Creative Biolabs are meticulously isolated from healthy human biliary tract tissue. These primary cells maintain native physiological features and surface biomarkers, making them an indispensable in vitro tool for barrier integrity assays and drug absorption studies.
Place the cryovial immediately into a 37°C water bath and agitate gently until only a small ice crystal remains (typically within 1 minute). Dilute rapidly with pre-warmed complete growth medium and centrifuge at low speed to remove the cryoprotectant.
These primary cells maintain robust growth and functional characteristics for 3-5 passages under optimal culture conditions. Using early-passage cells ensures high biological relevance in in vitro assays.
Each lot of HLA-typed Human Biliary Primary Epithelial Cells undergoes strict quality control testing, including sterility screening (free of bacteria, fungi, and mycoplasma), cell viability verification, and specific biomarker characterization.
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