How should these cells be thawed to ensure optimal viability?

Place the cryovial immediately into a 37°C water bath and agitate gently until only a small ice crystal remains (typically within 1 minute). Dilute rapidly with pre-warmed complete growth medium and centrifuge at low speed to remove the cryoprotectant.

What is the typical passage recommendation for these primary cells?

These primary cells maintain robust growth and functional characteristics for 3-5 passages under optimal culture conditions. Using early-passage cells ensures high biological relevance in in vitro assays.

Can Creative Biolabs provide donor information or customized cell lot matching?

Yes. Upon request, Creative Biolabs can provide detailed donor background information (such as age, gender, and health status) and offers custom lot reservation or tailored isolation batches for longitudinal studies.

HLA-typed Human Colon Primary Epithelial Cells (CAT#: DBFF-0926-HX73)

Datasheet

Overview

Derived from human colon tissue, HLA-typed Human Colon Primary Epithelial Cells preserve original tissue-specific functionalities. Creative Biolabs delivers these cells cryopreserved at early passages to ensure optimal biological performance for advanced in vitro modeling and pathogen interaction studies.

Quantity:

* For Research Use Only. Not For Clinical Use.

Specifications

Species

Human

Organ/Tissue

Colon

Source

Healthy human donors

Cell Type

HLA-typed human primary cells

Cell Status

Normal

Format

Cryopreserved

Product details

Quality Control

Negative for mycoplasma, bacteria, fungi, HIV, HBV, and HCV.

Applications

HLA matching and mismatch studies, immune response research, cell and gene therapy research, drug immunogenicity studies, and disease modeling.

Product properties

Storage

Liquid nitrogen

Shipping

Dry ice

FAQs

How should these cells be thawed to ensure optimal viability?
Place the cryovial immediately into a 37°C water bath and agitate gently until only a small ice crystal remains (typically within 1 minute). Dilute rapidly with pre-warmed complete growth medium and centrifuge at low speed to remove the cryoprotectant.
What is the typical passage recommendation for these primary cells?
These primary cells maintain robust growth and functional characteristics for 3-5 passages under optimal culture conditions. Using early-passage cells ensures high biological relevance in in vitro assays.
Can Creative Biolabs provide donor information or customized cell lot matching?
Yes. Upon request, Creative Biolabs can provide detailed donor background information (such as age, gender, and health status) and offers custom lot reservation or tailored isolation batches for longitudinal studies.

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For Research Use Only | Not For Clinical Use