B Cell Engineering Service by Editing at CCR5 Safe Harbor Locus

Services

Genome Editing at CCR5 Other Locus Editing One-stop Service Custom Project

Genomic safe harbors (GSHs) are identified sites of safe integration of the transgene. Three widely used human GSH sites include adeno-associated virus site 1 (AAVS1), chemokine (CC motif) receptor 5 (CCR5), and Rosa26. CCR5 encodes the major co-receptor for HIV-1. As a common site of gene insertion, the CCR5 locus is not transcriptionally active in human B cells. Therefore, the CCR5 locus is one of the ideal sites for B cell engineering.

Genome Editing Service at CCR5 Locus

Creative Biolabs aims to engineer the next generation of cellular therapies using gene editing. We are dedicated to developing engineered B cells to stably produce therapeutic antibodies and proteins. Careful design of gene editing tools can improve the specificity, efficacy, and safety of gene and cell therapies. Our B cell engineering service specifically transfers your gene of interest into the CCR5 GSH site in human chromosome 3 via CRISPR/Cas9-mediated homologous recombination or other genomic editing technologies. Our engineered B cells have wide applications in therapeutics, industry, and basic research.

Fig.2 Targeted Transgene Insertion into the CCR5 Locus by CRISPR/Cas9. Fig.1 Targeted Transgene Insertion into the CCR5 Locus by CRISPR/Cas9. (Creative Biolabs)

Genome Editing Service at Other Safe Harbor Locus

Only a small number of GSH sites for targeted transgene insertion are in wide use in human cells. Focusing on state-of-the-art genomic editing technologies, scientists at our labs have rich expertise and experience in cell engineering services. We have developed an efficient system to deliver CRISPR/Cas9 for the insertion of your gene of interest into CCR5. In addition to the CCR5 site, we also provide other selections of GSH sites including:

Editing at AAVS1 Locus

Editing at Rosa26 Locus

Editing at GAPDH Locus

One-stop B Cells Engineering Service

Creative Biolabs provides researchers with a one-stop service to engineering B cells by CRISPR/Cas9-based editing methods—from experimental design to cell line development and validation. We offer affordable, comprehensive custom B cell engineering service with a fast turnaround time to meet the exact requirement of your projects. The workflow of CRISPR/Cas9-based B cell engineering is as follows.

Fig.3 Experimental workflow of B cell engineering by CRISPR/Cas9. Fig.2 Experimental workflow of B cell engineering by CRISPR/Cas9. (Creative Biolabs)

Advantages

Expertise in primary B cells Culture

High Efficiency Genome Editing

Achieve multiplex gene editing

Collaborative project management

Start Your Custom Gene Editing Project

Creative Biolabs has developed a one-stop CRISPR/Cas9-based platform for B cell engineering. Our dedicated scientific team will work collaboratively with you to design and build the most optimized solution for your project. The following is some information about our service.

Turnaround Time

It depend on the recovery of the cells, the proliferation rate and transfection efficiency of the cells, and the validation assays requested by the customer.

Service Deliverable

We will provide engineered B cells, sequence analysis, and certificate of analysis.

Other Downstream Sevices

Upon request, we provide additional services to further evaluate the target gene editing at the protein level using Western Blot, ELISA, or related functional assays.

If you are interested in our services, please feel free to contact us to discuss your project.