B Cell Engineering Service by Editing at AAVS1 Safe Harbor Locus

Genome Editing Service at AAVS1 Safe Harbor Locus

CRISPR-Cas9 technology has been used in many broad-based applications such as therapeutics, industry, and basic research. Creative Biolabs provides B cell a comprehensive service to establish gene-modified B cells with specific genomic editing events at a safe harbor locus. Our dedicated team has developed a CRISPR system that is designed to integrate a gene of interest or any other genetic element into the AAVS1 safe harbor site in human chromosome 19 via homologous recombination. Insertion of an expression cassette into the AAVS1 safe-harbor site can be used to introduce a transgene for overexpression and rescue studies without disrupting the expression of endogenous genes. These modified B cells can produce specific therapeutic proteins needed for a specific disease through precision genomic editing.

Fig.1 Genomic editing at the AAVS1 locus by CRISPR/Cas9. (Creative Biolabs)

One-stop B Cells Engineering Service

Creative Biolabs provides researchers with a one-stop service for engineering B cell—from experimental design to cell line development and validation. We offer tools and solutions for every step in the B cell engineering workflow. With decades of experience in cell engineering, our in-house team delivers unique solutions to support even the most demanding projects. Save yourself time and hassle and have Creative Biolabs handle all aspects of your B cell engineering workflow, from start to finish. The workflow of B cell engineering is as follows.

Fig.2 Experimental workflow of cell engineering by CRISPR/Cas9. (Creative Biolabs)

Genome Editing Service at Other Safe Harbor Locus

In addition to AAVS1 safe harbor site, we also provide other selections of safe harbor sites including

• Editing at CCR5 safe harbor locus
• Editing at Rosa26 safe harbor locus
• Editing at GAPDH locus

Service Deliverables

Creative Biolabs offers several levels of affordable custom services for designing, creating, and validating engineered B cells by a CRISPR/Cas9-based genome editing tool. Partner with us to get reliable gene-edited B cells:

• Engineered B cell lines
• Sequence analysis
• Certificate of analysis

Any requirements please contact us.

Published Data

Data: CRISPR/Cas9 editing of primary human B cells at AAVS1 locus.

• Technology: CRISPR/Cas9
• Host cells: primary human B cells
• Delivery method: electroporation
• Cargo: CRISPR/Cas9 ribonucleoprotein
• Donor vector: AAV6
• Insertion site: the AAVS1 locus
• Insertion gene: GFP

Fig.3 A schematic strategy and the efficiency of CRISPR/CAS9 editing in primary human B cells. (Johnson, et al., 2018)

1. Johnson, M.J.; et al. Engineering of primary human B cells with CRISPR/Cas9 targeted nuclease. Scientific Reports. 2018, (1): 12144.

Related Sections:

• Editing at CCR5 Safe Harbor Locus
• Editing at Rosa26 Safe Harbor Locus
• Editing at GAPDH Locus