N-(2-Hydroxyethyl)maleimide Heterobifunctional Crosslinker (CAT#: OLY-1023-YS7)

Description

This heterobifunctional crosslinker possesses a sulfhydryl-reactive maleimide group and a terminal primary hydroxyl group. The maleimide group reacts specifically with thiols (e.g., cysteine residues) at pH 6.5-7.5 to form a stable thioether bond. The terminal hydroxyl group is less nucleophilic than an amine but offers distinct conjugation possibilities. It is primarily used to prepare thiol-reactive surfaces by immobilizing the linker via its hydroxyl group, or to functionalize cysteine-containing molecules with a hydroxyl handle for further modification or enzymatic recognition (e.g., phosphorylation).

Molecular Weight

141.13

Purity

>98.0%

Formula

C₆H₇NO₃

Product Type

Heterobifunctional Crosslinkers

Group 1

Maleimide

Group 2

OH

Form (20 deg.C)

Solid

Storage Condition

-20°C

Molecular Structure