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Bispecific Intrabody Generation Service

Based on years of experience in antibody discovery and manufacture, Creative Biolabs has established a second to none bispecific antibodies (BsAbs) generation platform. With our comprehensive platform, we are proud to offer bispecific intrabody generation services in a timely and cost-effective manner.

Intrabody

Antibodies enable to bind almost any molecule with high affinity and specificity, offering potent biotechnological tools for diagnostic and therapeutic applications. Progresses in recombinant DNA technology have promoted the manipulation of the antibody genes, thus design, cloning, expression, and use of single-chain antibodies have become routine procedures in protein engineering. The potential of single-chain antibody fragments (scFv) for intracellular applications, named as “intrabodies,” has been studied in many laboratories. Intrabodies have been applied to target proteins in a singular fashion. Bispecific and tetravalent antibody fragments are able to promote and expand the inhibitory potential of intrabodies via displaying raised apparent affinity for their antigen and via being more efficient at inhibiting protein function or intracellular trafficking. Intrabodies stand for a powerful alternative to approaches of gene inactivation that target at the level of DNA or mRNA, for example, antisense, zinc finger proteins, targeted gene disruption, or the relatively new RNA interference.

Diagram of Bispecific intrabody structure.

Figure 1. Diagram of Bispecific intrabody structure.

Bispecific Intrabody

A bispecific intrabody has two identical immunoglobulin chains paired via its recombinant Fc domains. Meanwhile, two pairs of single chain Fvs (scFvs) with distinct specificity are linked to each end of the Fc domain respectively. Since the component unit for dimerization is uniform, this strategy significantly simplifies the generation process of bispecific products. Furthermore, as each component unit is bispecific and bivalence, the homodimer product is not only bispecific but also tetravalence. These properties make bispecific intrabody a leading candidate format for both drug development and academic research.

A primary application for intrabodies lies in functional genomics. As the number of proteins with unknown function and their interactions with other proteins steadily rise, bispecific intrabody could offer an attractive proteomic tool to analyze these networks. Moreover, bispecific intrabody is used to study linked regulatory pathways or block redundant pathways. In addition, bispecific intrabody may extend the therapeutic applicability of intrabodies.

With our well-established bispecific intrabody generation platform, the experienced scientists here at Creative Biolabs are dedicated to help you develop unique bispecific intrabody. We also provide other various services regarding BsAbs development. Please feel free to contact us for more information and a detailed quote.

References

1. Jendreyko, N.; et al. Intradiabodies, bispecific, tetravalent antibodies for the simultaneous functional knockout of two cell surface receptors. Journal of Biological Chemistry. 2003, 278(48): 47812-47819.
2. Butler, D. C.; Messer, A. Bifunctional anti-huntingtin proteasome-directed intrabodies mediate efficient degradation of mutant huntingtin exon 1 protein fragments. PloS one. 2011, 6(12): e29199.

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