Aptamers can bind to the receptors on the cell membrane and mediate themselves or conjugated nanoparticles to enter into cells. Thus, aptamers can be served as ideal targeting ligands for drug delivery. With an extensive line of services to support your projects, Creative Biolabs is the best choice for high-quality, cost-effective collaborating / outsourcing options for your research programs. We can meet customer’s unmet needs with different kinds of our aptamer services. Our technology has a shorter development time compared to traditional methods of aptamer-targeted drug development and can be used to increase the specificity and improve the delivery of existing candidates resulting in the next generation of pharmaceuticals with improved pharmacodynamics and pharmacokinetics.
Aptamers are capable of binding to receptor molecules, which could lead to aptamer-based delivery systems and the usage of chemical tags alongside available chemical strategies to improve cellular uptake. Aptamer-mediated drug delivery has shown potential as a cancer treatment strategy. The primary considerations in the design of a targeted drug delivery system are to promote the accumulation of chemotherapeutic agents in the tumor tissue and overcome drug resistance. Ligands can guide nanocarriers to tumors and facilitate the transport of these nanocarriers into tumor cells, resulting in cell death. Depending on the compositions and preparation approaches, aptamer-targeted drug delivery systems can be divided into the following two main categories.
Advantage: Good chemical stability, isotropic properties
Disadvantage: Low drug loading, complex and costly procedures
Advantage: High loading capacity
Disadvantage: Unpredictable risks, complex and costly procedures
Figure.1 Nanomaterials applied in aptamer-targeted drug delivery systems.
The cell membrane is the barrier for the import and export of DNA/RNA to and from the cell. Cell membrane proteins from bacteria, viruses, and mammalian cells play an important role in pathogenesis and may be potential targets for the next generation of aptamers. With the basic developments in SELEX, different strategies have been utilized for generating cell-internalizing aptamers, such as whole-cell SELEX, protein SELEX, tissue-specific SELEX, and in vivo SELEX. Cell-SELEX is a straightforward way to generate internalizing aptamers because it involves direct interaction with whole cells and the selection of aptamer exclusively depends on the surface antigens. Besides, internalizing aptamers can also be developed via tissue penetration using the SELEX strategies. Oligonucleotide library is injected into the tail of the mouse. The targeted tissue will be dissected for the isolation of bound molecules.
Fig.2 Illustration for steps involved in the cell-specific (A) and in vivo (B) SELEX process. (Gopinath, 2016)
Whether you have a cell type of interest or have identified a specific cell surface target, the first step in aptamer-mediated drug delivery is the discovery of selective aptamers. Creative Biolabs has discovered aptamers to cells, cell surface markers, small molecule drugs, and drug metabolites. Our advanced multiplex selection enables simultaneous discovery of selective aptamers to a panel of positive and negative targets, reducing the overall time and cost of aptamer discovery and improving aptamer selectivity. If you are interested in aptamer development for drug delivery, please feel free to contact us for more information.
Reference
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