Introduction of TRHR
TRHR, a G protein-coupled receptor, is encoded by TRHR gene. It binds to the tripeptide thyrotropin releasing hormone (TRH). It has been extensively studied during the past few decades because it offers numerous possibilities for therapeutic applications. TRHR is found in the brain, and when bound with TRH, it acts to increase intracellular inositol trisphosphate through phospholipase C. Some studies reported TRH and TRHR can cause a variety of thyroidal or non-thyroidal effects, and they are regarded as biomarkers and drug candidates.
|Basic Information of TRHR|
|Protein Name||Thyrotropin-releasing hormone receptor|
|Organism||Homo sapiens (Human)|
Function of TRHR Membrane Protein
TRH receptors (TRHRs) are seven transmembrane domain GTP (G)-protein coupled receptors (GPCR) that are members of group A7 of the rhodopsin-like receptor family. TRHR has been studied in detail in rodents, where two subtypes are identified and named as TRHR1 and TRHR2. TRHR plays a vital role in many biological functions, such as resisting depression, reducing anxiety and improving learning and memory. Lots of research demonstrate that TRHR is associated with several diseases, such as diabetes, breast cancer, depression, spinal cord injury, and amyotrophic lateral sclerosis. Regulation of TRH production in the hypothalamus, patterns of expression of TRH and its receptor in the body, could be beneficial for clinical therapy.
Fig.1 The hypothalamus, pituitary, and the thyroid gland.
Application of TRHR Membrane Protein in Literature
This article analyzes the proliferation of receptors in the cell membranes of HEK293 cells which steadily express TRH receptor by fluorescence recovery after photobleaching (FRAP), the TRH receptor is markedly reduced upon TRH binding. Meanwhile, the same results also find in Gq/11α, Gβ, β-arrestin2, and phospholipase Cβ1, which suggests that these proteins may also play distinct roles in THR receptor-mediated signaling.
Authors analyze the expression level of three types of cloned bullfrog TRHRs by reverse transcription-PCR. The data reveals TRHR3 mRNA is expressed in the anterior lobe and that the signals reside mostly in the prolactin (PRL) cells, which suggests TRHR3 mediates the action of TRH on the PRL cells to induce the release of PRL that is prerequisite for growth and metamorphosis in amphibians.
Authors in this group investigate the effects and underlying mechanism of thyrotropin releasing hormone (TRH) on colonic contractile disorders induced by chronic water avoidance stress (WAS). These results show TRH may be related to the dysmotility induced by chronic stress.
This article determines the ability of JAK4D to bind to TRH receptors in human brain and to evaluate its neuropharmacological effects in neurodegenerative animal models. The result shows that JAK4D is an important tool for investigating the hitherto-unidentified central TRH receptor subtype reported herein and an attractive therapeutic candidate for neurodegenerative disorders.
This article evaluates how the TRH receptors engage novel signaling pathways in PVT neurons, and the effect of postsynaptic intracellular CB1 and CB2 receptors on TRPC-like pathway. These results indicate that CB1 and CB2 suppress the TRPC-like channel opening.
TRHR Preparation Options
To obtain the soluble and functional target protein, the versatile Magic™ membrane protein production platform in Creative Biolabs enables many flexible options, from which you can always find a better match for your particular project. Aided by our versatile Magic™ anti-membrane protein antibody discovery platform, we also provide customized anti-TRHR antibody development services.
As a forward-looking research institute as well as a leading custom service provider in the field of membrane protein, Creative Biolabs has won good reputation among our worldwide customers for successfully accomplishing numerous challenging projects including generation of many functional membrane proteins. Please feel free to contact us for more information.