3D Spheroid and Endothelial Cell Co-Culture Model for Immuno-Oncology Drug Discovery and Development

Traditional cancer research focused on analyzing the biological status of tumor cells in vitro, which has hindered the extension of various studies to the in vivo environment. The inherent flaws in these models have resulted in frequent disappointment in the translation/expansion of the principles behind the anti-tumor compounds or procedures used toward clinical outcomes.

Endothelial Cells in Tumor

Tumor cells thrive alongside endothelial cells in their microenvironment, assuming a pivotal role in the regulation of the critical process of angiogenesis, which represents one of the primary indicators of tumor genesis. The reciprocal interplay between tumor cells and endothelial cells facilitates modifications in the EC24 gene expression repertoire and its activation state, consequently exerting direct influence over the pathological manifestations of the tumor.

Fig 1. Illustration showing the isolation of tumor cells from a patient surgical sample, and assembly of tumor spheroids or TC:EC hybrid spheroids.¹

Creative Biolabs' 3D Tumor Spheroid and Endothelial Cells Co-Culture Model

Compared to traditional 2D in vitro culture, the 3D tumor spheroid cell model is a more suitable model for clinically relevant tumor disease research. We rapidly create tissue models that replicate the tumor microenvironment through the self-assembly of cell aggregates. The implantation of endothelial cells aids in studying the interplay and angiogenesis processes of cancer cells.

Key Features

Endothelial Cell Implantation

The complex interconnections and crosstalk between endothelial cells and cancer cells initiate angiogenesis and mimic the drug resistance of pathological conditions.

Optional Complex Models

Healthy and cancerous epithelial cells cultured in a 3D basement membrane-like matrix will self-organize into spherical, polarized structures.

Stable Spheroid Formation

Through optimized cell spheroid production processes, our model is more suitable for controlled experimental operations and replication.

Reliable biomimetic properties

Our 3D tumor cell co-culture spheroids exhibit higher proliferation rates, a greater propensity for spheroid formation, increased expression of matrix metalloproteinases, and higher chemotherapeutic resistance.

Personalized medicine

In addition to improving tissue structure, we can also construct models using almost any primary human tissue, stromal cells, or matrices, thus eliminating the heterogeneity of tumor diseases for any impact on your research.

What Else We Provide?

By introducing the precise ratio of tumor cells and various stromal cells, we have developed numerous 3D spheroid models listed below for immune-oncology research.

Creative Biolabs is confident that our expertise and dedication will be instrumental in enhancing the outcomes of your research. We apply 3D biology theories to provide the most physiologically relevant research models and related services for scientists worldwide. For more information, please contact our team of experts.

Related Sections:

• Tumor Organoid for Immuno-Oncology Drug Discovery and Development
• 3D Spheroid Model for Immuno-Oncology Drug Discovery and Development