Tumor Organoid Monoculture Model for Immuno-Oncology Drug Discovery and Development

Creative Biolabs offers the tumor organoid monoculture model (TOMM), aiming to provide reliable, reproducible, and manageable research tools for immunotherapy drug discovery and development. This model is designed to accelerate the research and development of novel immunotherapeutic approaches. The use of this model helps to better understand the interaction between tumors and the immune system, optimizing drug screening and evaluation.

Comprehensive Interpretation of TOMM

The TOMM refers to the construction of tumor organoids (TOs) using a single tumor sample and culturing and expanding the cells in vitro. This model is typically created by dissociating tumor tissues into individual cells and culturing them under appropriate conditions to allow for self-organization and the formation of three-dimensional cellular structures.

Advantages of the TOMM

• Preservation of tissue heterogeneity: The TOMM can preserve the cellular heterogeneity of the original tumor sample, including the presence of different subtypes, cell types, and gene expression patterns. This helps to more accurately replicate the complexity of the original tumor.
• Ease of expansion: Compared to large-scale animal models or xenograft models, the TOMM can be cultured and expanded over an extended period, allowing for the generation of a larger number of cells from limited tumor samples.
• Ease of preservation: Like regular cell cultures, the TOMM can be cryopreserved and revived, facilitating subsequent experiments and applications. This model provides researchers with a controlled and reproducible in vitro platform.
• Assessment of immunotherapy response: The TOMM allows for the evaluation of the cytotoxic effects of different immunotherapy drugs on tumor cells within different TOs. This contributes to the optimization of the design and development of immunotherapy drugs.

Application Example of TOMM

Title: Drug Repurposing Screening and Mechanism Analysis Based on Human Colorectal Cancer Organoids

Organoid Model: Colorectal cancer organoids derived from tumor tissues of colorectal cancer patients

Objective: To screen for potential therapeutic candidates with value in the treatment of colorectal cancer

Methods:

Fig.1 Experimental workflow of drug screening based on human colorectal cancer organoids.¹

Conclusion: The authors successfully screened potential candidate drugs targeting colorectal cancer through high-throughput drug screening based on colorectal cancer organoids. They combined computer prediction analysis and validation in a mouse animal model. Furthermore, they utilized transcriptome sequencing technology to reveal the mechanisms and modes of action of these candidate drugs.

TOMM Supply at Creative Biolabs

As a company specializing in biopharmaceutical research and development services, Creative Biolabs provides support in the following areas based on the needs and research objectives of our clients:

1. Tumor biology research
2. Discovery of targets for tumor immunotherapy
3. Screening of immune-oncology drugs
4. Evaluation of immune-oncology drugs
5. Research on tumor drug resistance
6. Drug interaction studies

If you have any questions regarding the TOMM or other related services, or if you would like to inquire for more detailed information, please contact us directly. Our professional team will be more than happy to answer your questions and provide relevant solutions.

Related Sections:

• Tumor Organoid for Immuno-Oncology Drug Discovery and Development
• 3D Spheroid Model for Immuno-Oncology Drug Discovery and Development