Creative Biolabs is offering the most comprehensive services for antibody development projects. With strict regulation and effective execution, we are dedicated to providing the most valuable solutions to complete your projects.
HighlightsCreative Biolabs is offering the most comprehensive services for antibody development projects. With strict regulation and effective execution, we are dedicated to providing the most valuable solutions to complete your projects.
HighlightsCreative Biolabs has established a broad range of platforms for developing novel antibodies or equivalents. These cutting-edge technologies enable our scientists to meet your demands from different aspects and tailor the most appropriate solution that contributes to the success of your projects.
HighlightsWith deep understanding in antibody-related realms and extensive project experience, Creative Biolabs offers a variety of references to help you learn more about our capacities and achievements, including infographic, flyer, case study, peer-reviewed publications, and all kinds of knowledge that can assist your projects. You are also welcome to contact us directly for more specific solutions.
HighlightsGet a real taste of Creative Biolabs, one of the most professional custom service providers in the world. We are committed to providing highly customized comprehensive solutions with the best quality to advance your projects.
Highlights| Protein Name | Adhesion G protein-coupled receptor F5 |
| Gene Name | ADGRF5 |
| Uniprot | Q8IZF2 (Human); G5E8Q8 (Mouse) |
| Synonym | GPR116; KPG_001; G-protein coupled receptor 116; Ig-Hepta homolog; probable G-protein coupled receptor 116 |
| Background | ADGRF5, also known as G-protein coupled receptor 116 (GPR116), is a protein encoded by the human GPR116 gene. It is a member of the adhesion class of GPCRs (AdGPCRs), which share extracellular domains containing highly conserved GPCR autoproteolysis-inducing (GAIN) domains. During synthesis of ADGRF5, intracellular autoproteolytic processing of nascent ADGRF5 proteins within the GAIN domain generates a mature protein, consisting of an N-terminal fragment (NTF) that is noncovalently linked to the C-terminal fragment (CTF), consisting of a 7 transmembrane (7TM) domain and a cytoplasmic domain. |
Creative Biolabs offers comprehensive range of membrane protein products empowers your unique research needs.
| CAT# | Product Name | Expression System | Protein Length | Solubilizing Agents |
| S01YF-1023-KX312 | NativeExtract™ Human ADGRF5 Membrane Protein (Full length, Super Nanodisc) | HEK293 cell | Full length | Native Nanodisc |
| MP0439X | MemDX™ Membrane Protein Human ADGRF5 (Adhesion G protein-coupled receptor F5) for Antibody Discovery | Wheat germ cell-free | Full length | Liposome |
| Please for more products or customization | ||||
Ph***ix: A cost-effective solution for rigorous GPCR research.
03/May/2023
Ca***an: Highly recommend for urgent projects.
12/Dec/2023
We provide tailored solutions to meet your specific requirements. Please for more services.
Gene Knockout
Creative Biolabs offers gene knockout services. ADGRF5, a critical adhesion G protein-coupled receptor implicated in cellular adhesion and oncogenic signaling, represents a prime candidate for gene knockout. By designing single-guide RNAs complementary to exonic regions of ADGRF5, researchers can achieve precise inactivation of the locus, effectively ablating its tumor-promoting activity in preclinical models. Creative Biolabs' proprietary gene editing optimization platform ensures high-specificity targeting, minimizing off-site edits—a cornerstone for clinical translation.
AAV-Driven Overexpression for Tissue Regeneration
Creative Biolabs offers AAV-driven overexpression services for tissue regeneration. Adeno-associated virus (AAV) vectors engineered with tissue-specific promoters enable spatially controlled ADGRF5 overexpression, particularly valuable in regenerative contexts. In a murine model of pulmonary fibrosis, AAV9 vectors carrying a constitutive ADGRF5 expression cassette under the control of a lung epithelium-specific promoter achieved sustained transgene expression, attenuating collagen deposition and restoring alveolar architecture. Creative Biolabs' expertise in AAV serotype selection and promoter design ensures optimal delivery to target tissues, a critical consideration for mitigating systemic toxicity.
RNA Interference
Creative Biolabs offers RNA interference services. Small interfering RNA (siRNA) and short hairpin RNA (shRNA) platforms offer transient yet potent ADGRF5 suppression, ideal for acute therapeutic scenarios. Lipid nanoparticle-encapsulated siRNAs targeting conserved regions of ADGRF5 mRNA have shown efficacy in reducing tumor burden in hepatocellular carcinoma xenografts, with a 60% reduction in metastatic nodules observed post-treatment. Creative Biolabs' RNAi optimization services, including chemical modification and biodistribution profiling, ensure stability and tissue specificity.
Creative Biolabs offers comprehensive and innovative services to drive the development of ADGRF5 cell therapy. Please for more services.
CAR-T Cells Targeting ADGRF5-Positive Malignancies
Creative Biolabs offers CAR-T cells development services targeting ADGRF5-positive malignancies. ADGRF5's cell surface expression in select cancers positions it as a viable target for CAR-T cell therapies. Second-generation CAR constructs incorporating anti-ADGRF5 single-chain variable fragments (scFvs) coupled with 4-1BB co-stimulatory domains have demonstrated potent cytolytic activity against ADGRF5+ glioblastoma stem cells in vitro, achieving >80% target cell clearance. Creative Biolabs' CAR-T development pipeline includes scFv humanization and cytotoxicity profiling, essential for minimizing on-target/off-tumor effects.
iPSC-Derived ADGRF5-Reporter Lines for Drug Screening
Creative Biolabs offers iPSC-derived ADGRF5-reporter lines development services. Engineering iPSCs with ADGRF5 promoter-driven fluorescent reporters enables high-throughput screening of modulators. A recent study utilizing such lines identified a novel small molecule agonist that upregulated ADGRF5 expression by 3.5-fold, enhancing epithelial barrier function in inflammatory bowel disease organoids. Creative Biolabs' stem cell engineering services, ensure reporter line fidelity—critical for reproducibility.
Ex Vivo Gene-Edited HSCs for Immune Reconstitution
Creative Biolabs offers ex vivo gene-edited HSCs development services. Ex vivo editing of HSCs to overexpress ADGRF5 could potentiate immune surveillance in immunodeficiency states. Lentiviral transduction of HSCs with an ADGRF5 expression construct enhanced myeloid cell adhesion and pathogen clearance in a neutropenia model, reducing sepsis mortality by 40%. Creative Biolabs' lentiviral production and HSC transduction protocols ensure clinical-grade output.
The multifaceted roles of ADGRF5 in disease pathogenesis demand a partner with deep technical mastery and translational acumen. Creative Biolabs' vertically integrated services—spanning gene editing, viral vector engineering, and cell therapy development—are tailored to de-risk your ADGRF5-targeted programs. Our team delivers end-to-end solutions underpinned by two decades of industry leadership. Please feel free to to schedule a technical consultation and accelerate your pipeline with precision-driven science.
All listed services and products are For Research Use Only. Do Not use in any diagnostic or therapeutic applications.
