Human respiratory syncytial virus (RSV) is the major cause of lower respiratory tract infections in infants leading to hospitalization and an increased risk factor for asthma development. It is reported that the immune system plays an important role in the pathogenesis of RSV disease and RSV is associated with the exacerbation of airway inflammation. Creative Biolabs provides B cell engineering services by different genome editing methods to manufacture antibodies to defend against the invasion of RSV.
Creative Biolabs engineers primary B cells with precise genome editing techniques, which replace endogenously encoded antibodies of primary human B cells with antibodies that target RSV. The engineered B cells enable immunological memory and clonal selection that may contribute to addressing viral variability and to counteracting viral escape.
Fig.1 B cell engineering service for anti-RSV antibody delivery. (Creative Biolabs)
Paper Title: B Cells Engineered to Express Pathogen-specific Antibodies Protect Against Infection
Technology: CRISPR/Cas9-based Genome Editing
Therapeutic Antibody: Anti-RSV Antibody
Journal: Science Immunology
IF: 30.63
Published: 2019
Background: Effective vaccines for RSV are not available despite decades of research. This research developed an alternative method, a genetic engineering strategy, in which CRISPR-Cas9 was used to replace endogenously encoded antibodies with antibodies targeting RSV in primary human B cells. The engineered antibodies were expressed efficiently in primary B cells under the control of endogenous regulatory elements, which maintained normal antibody expression and secretion.
Results: Using engineered mouse B cells, a single transfer of B cells engineered to express an antibody against RSV resulted in potent and durable protection against RSV infection in RAG1-deficient mice.
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B Cell Engineering Service for Anti-COVID-19 Antibody Delivery |
| Creative Biolabs provides the B cell engineering service to manufacture antibodies to defend against the invasion of the COVID-19 virus. Please click to know more. | |
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B Cell Engineering Service for Anti-HIV Antibody Delivery |
| Creative Biolabs provides the B cell engineering service to manufacture antibodies to defend the invasion of HIV virus. Please click to know more. | |
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B Cell Engineering Service for Anti-Influenza Virus Antibody Delivery |
| Creative Biolabs provides the B cell engineering service to manufacture antibodies to defend against the invasion of influenza virus. Please click to know more. | |
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B Cell Engineering Service for Anti-EBV Antibody Delivery |
| Creative Biolabs provides B cell engineering services to manufacture antibody by to defend against the invasion of EVB. Please click to know more. |
Q1. What techniques can also be applied to engineer B cells for RSV therapy?
A: BCR editing has been applied to introduce antibodies specific to RSV. For more details for this information, please contact us and ask for the document.
Q2. What methods will be used in the assessment experiment?
A: Flow cytometry and ELISA are frequently used methods. The specific experiments can be adjusted at any time to meet the requirements of your project.
With a team of scientists who come from different research backgrounds,Creative Biolabs guides you all the way from project inception to high-quality results. For more B cell engineering information, please visit the unit of B Cell Engineering Service for Therapeutic Antibody Delivery. If you have interest, please do not hesitate to contact us.
