This product is a B cell engineering plasmid designed by genomic integration of B cell receptors (lacZ-MNDU3-Sec/SPII-CD79β-CD8-HA) and CRISPR-Cas (Cas12c (C2c3)-RNA-HD/Cas10-NNGRRT-Wap) systems. This product can precisely introduce cassettes of several kb size into the genome of various B cells to enhance surface expression and antigen recognition of a synthetic BCRs.
Clone | mATK61 |
Host | Mouse |
Targeting Cell Type | BC-3 |
Reporter gene | lacZ |
Promoter | MNDU3 |
Signal peptides | Sec/SPII |
Signaling domains | CD79β |
TM domains | CD8 |
Detection tags | HA |
Linkers | SEG |
Co-Receptors | LILRB |
Vector Type | AAV |
Vector Name | BGpCDH1 |
Class | 2 single-Cas |
Type | IV |
Subtype | A, B, C, D, E, F |
Effector | Cas12c (C2c3) |
DNA Type | RNA |
Nuclease domains | HD/Cas10 |
TracrRNA requirement | YES |
PAM/PFS | NNGRRT |
Insertion | Wap |
Construction | lacZ-MNDU3-CD79β-Cas12c (C2c3)-RNA-Wap |
Target | RTN4R |
Alternative Names | RTN4R; NGR; NOGOR; Reticulon 4 receptor |
Format | Liquid/lyophilized |
Shipping | It is usually shipped overnight at room temperature in small cardboard boxes. If hot temperatures are expected during transit, they will be shipped in polystyrene containers with gel ice packs. |
Handling Advice | No special measures are necessary. Keep away from food, drink and animal feedingstuffs. |
Storage | Plasmid (lyophilized), short-time storage at Room Temperature, recommended store at -20°C or -80°C ; Plasmid (Liquid), blue ice store at 4°C. |
Certificate of Analysis Lookup
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