RTN4R mAb (mATK61) B Cell Engineering Specific Plasmid (CAT#: BGS-1223-CR2)

This product is a B cell engineering plasmid designed by genomic integration of B cell receptors (lacZ-MNDU3-Sec/SPII-CD79β-CD8-HA) and CRISPR-Cas (Cas12c (C2c3)-RNA-HD/Cas10-NNGRRT-Wap) systems. This product can precisely introduce cassettes of several kb size into the genome of various B cells to enhance surface expression and antigen recognition of a synthetic BCRs.

Datasheet

Specifications

Clone mATK61
Host Mouse
Targeting Cell Type BC-3
Reporter gene lacZ
Promoter MNDU3
Signal peptides Sec/SPII
Signaling domains CD79β
TM domains CD8
Detection tags HA
Linkers SEG
Co-Receptors LILRB
Vector Type AAV
Vector Name BGpCDH1
Class 2 single-Cas
Type IV
Subtype A, B, C, D, E, F
Effector Cas12c (C2c3)
DNA Type RNA
Nuclease domains HD/Cas10
TracrRNA requirement YES
PAM/PFS NNGRRT
Insertion Wap
Construction lacZ-MNDU3-CD79β-Cas12c (C2c3)-RNA-Wap

Target Information

Target RTN4R
Alternative Names RTN4R; NGR; NOGOR; Reticulon 4 receptor

Product Properties

Format Liquid/lyophilized
Shipping It is usually shipped overnight at room temperature in small cardboard boxes. If hot temperatures are expected during transit, they will be shipped in polystyrene containers with gel ice packs.
Handling Advice No special measures are necessary. Keep away from food, drink and animal feedingstuffs.
Storage Plasmid (lyophilized), short-time storage at Room Temperature, recommended store at -20°C or -80°C ; Plasmid (Liquid), blue ice store at 4°C.
COA

Certificate of Analysis Lookup

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