This product is a B cell engineering plasmid designed by genomic integration of B cell receptors (RFP-mPGK-Sec/SPIII-CD79α-CD28-MBP) and CRISPR-Cas (Cas13a (C2c2)-RNA-HD/Cas3-NNGRRT-Wap) systems. This product can precisely introduce cassettes of several kb size into the genome of various B cells to enhance surface expression and antigen recognition of a synthetic BCRs.
Clone | NOIRT2135 |
Host | Human |
Targeting Cell Type | Raji-Luc2 |
Reporter gene | RFP |
Promoter | mPGK |
Signal peptides | Sec/SPIII |
Signaling domains | CD79α |
TM domains | CD28 |
Detection tags | MBP |
Linkers | 22 Amino Acid Linker |
Co-Receptors | CD19 |
Vector Type | Lentivirus |
Vector Name | BGpCDH2 |
Class | 1 multi-Cas |
Type | III |
Subtype | A, B, C |
Effector | Cas13a (C2c2) |
DNA Type | RNA |
Nuclease domains | HD/Cas3 |
TracrRNA requirement | No |
PAM/PFS | NNGRRT |
Insertion | Wap |
Construction | RFP-mPGK-CD79α-Cas13a (C2c2)-RNA-Wap |
Target | RTN4R |
Alternative Names | RTN4R; NGR; NOGOR; Reticulon 4 receptor |
Format | Liquid/lyophilized |
Shipping | It is usually shipped overnight at room temperature in small cardboard boxes. If hot temperatures are expected during transit, they will be shipped in polystyrene containers with gel ice packs. |
Handling Advice | No special measures are necessary. Keep away from food, drink and animal feedingstuffs. |
Storage | Plasmid (lyophilized), short-time storage at Room Temperature, recommended store at -20°C or -80°C ; Plasmid (Liquid), blue ice store at 4°C. |
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