RTN4R mAb (NOIRT2135) B Cell Engineering Specific Plasmid (CAT#: BGS-1223-CR5)

This product is a B cell engineering plasmid designed by genomic integration of B cell receptors (RFP-mPGK-Sec/SPIII-CD79α-CD28-MBP) and CRISPR-Cas (Cas13a (C2c2)-RNA-HD/Cas3-NNGRRT-Wap) systems. This product can precisely introduce cassettes of several kb size into the genome of various B cells to enhance surface expression and antigen recognition of a synthetic BCRs.

Datasheet

Specifications

Clone NOIRT2135
Host Human
Targeting Cell Type Raji-Luc2
Reporter gene RFP
Promoter mPGK
Signal peptides Sec/SPIII
Signaling domains CD79α
TM domains CD28
Detection tags MBP
Linkers 22 Amino Acid Linker
Co-Receptors CD19
Vector Type Lentivirus
Vector Name BGpCDH2
Class 1 multi-Cas
Type III
Subtype A, B, C
Effector Cas13a (C2c2)
DNA Type RNA
Nuclease domains HD/Cas3
TracrRNA requirement No
PAM/PFS NNGRRT
Insertion Wap
Construction RFP-mPGK-CD79α-Cas13a (C2c2)-RNA-Wap

Target Information

Target RTN4R
Alternative Names RTN4R; NGR; NOGOR; Reticulon 4 receptor

Product Properties

Format Liquid/lyophilized
Shipping It is usually shipped overnight at room temperature in small cardboard boxes. If hot temperatures are expected during transit, they will be shipped in polystyrene containers with gel ice packs.
Handling Advice No special measures are necessary. Keep away from food, drink and animal feedingstuffs.
Storage Plasmid (lyophilized), short-time storage at Room Temperature, recommended store at -20°C or -80°C ; Plasmid (Liquid), blue ice store at 4°C.
COA

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.

Lot Number
! For Research Use Only | Not For Clinical Use

Related products