This product is a B cell engineering plasmid designed by genomic integration of B cell receptors (YFP-EFS-Tat/SPI-CD79α-CD28-CBP) and CRISPR-Cas (Cas12b (C2c1)-dsDNA-Stat5a) systems. This product can precisely introduce cassettes of several kb size into the genome of various B cells to enhance surface expression and antigen recognition of a synthetic BCRs.
Clone | NOIRT2335 |
Host | Human |
Targeting Cell Type | Farage |
Reporter gene | YFP |
Promoter | EFS |
Signal peptides | Tat/SPI |
Signaling domains | CD79α |
TM domains | CD28 |
Detection tags | CBP |
Linkers | GSAT Linker |
Co-Receptors | Siglec-G |
Vector Type | MMLV |
Vector Name | BGpCDH5 |
Class | 1 multi-Cas |
Type | III |
Subtype | C |
Effector | Cas12b (C2c1) |
DNA Type | dsDNA |
Nuclease domains | Unknown |
TracrRNA requirement | YES |
PAM/PFS | - |
Insertion | Stat5a |
Construction | YFP-EFS-CD79α-Cas12b (C2c1)-dsDNA-Stat5a |
Target | RTN4R |
Alternative Names | RTN4R; NGR; NOGOR; Reticulon 4 receptor |
Format | Liquid/lyophilized |
Shipping | It is usually shipped overnight at room temperature in small cardboard boxes. If hot temperatures are expected during transit, they will be shipped in polystyrene containers with gel ice packs. |
Handling Advice | No special measures are necessary. Keep away from food, drink and animal feedingstuffs. |
Storage | Plasmid (lyophilized), short-time storage at Room Temperature, recommended store at -20°C or -80°C ; Plasmid (Liquid), blue ice store at 4°C. |
Certificate of Analysis Lookup
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