RTN4R mAb (NOIRT2335) B Cell Engineering Specific Plasmid (CAT#: BGS-1223-CR3)

This product is a B cell engineering plasmid designed by genomic integration of B cell receptors (YFP-EFS-Tat/SPI-CD79α-CD28-CBP) and CRISPR-Cas (Cas12b (C2c1)-dsDNA-Stat5a) systems. This product can precisely introduce cassettes of several kb size into the genome of various B cells to enhance surface expression and antigen recognition of a synthetic BCRs.

Datasheet

Specifications

Clone NOIRT2335
Host Human
Targeting Cell Type Farage
Reporter gene YFP
Promoter EFS
Signal peptides Tat/SPI
Signaling domains CD79α
TM domains CD28
Detection tags CBP
Linkers GSAT Linker
Co-Receptors Siglec-G
Vector Type MMLV
Vector Name BGpCDH5
Class 1 multi-Cas
Type III
Subtype C
Effector Cas12b (C2c1)
DNA Type dsDNA
Nuclease domains Unknown
TracrRNA requirement YES
PAM/PFS -
Insertion Stat5a
Construction YFP-EFS-CD79α-Cas12b (C2c1)-dsDNA-Stat5a

Target Information

Target RTN4R
Alternative Names RTN4R; NGR; NOGOR; Reticulon 4 receptor

Product Properties

Format Liquid/lyophilized
Shipping It is usually shipped overnight at room temperature in small cardboard boxes. If hot temperatures are expected during transit, they will be shipped in polystyrene containers with gel ice packs.
Handling Advice No special measures are necessary. Keep away from food, drink and animal feedingstuffs.
Storage Plasmid (lyophilized), short-time storage at Room Temperature, recommended store at -20°C or -80°C ; Plasmid (Liquid), blue ice store at 4°C.
COA

Certificate of Analysis Lookup

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