This product is a B cell engineering plasmid designed by genomic integration of B cell receptors (CAT-EF1A-Sec/SPII-CD79β-CD8-Myc) and CRISPR-Cas (Cas12a (Cpf1)-ssRNA-RuvC-NGG-Csn) systems. This product can precisely introduce cassettes of several kb size into the genome of various B cells to enhance surface expression and antigen recognition of a synthetic BCRs.
Clone | NOIRT2337 |
Host | Human |
Targeting Cell Type | DS-1 |
Reporter gene | CAT |
Promoter | EF1A |
Signal peptides | Sec/SPII |
Signaling domains | CD79β |
TM domains | CD8 |
Detection tags | Myc |
Linkers | Flexible Linker |
Co-Receptors | FcγRIIB |
Vector Type | Lentivirus |
Vector Name | BGpCDH2 |
Class | 2 single-Cas |
Type | II |
Subtype | D |
Effector | Cas12a (Cpf1) |
DNA Type | ssRNA |
Nuclease domains | RuvC |
TracrRNA requirement | No |
PAM/PFS | NGG |
Insertion | Csn |
Construction | CAT-EF1A-CD79β-Cas12a (Cpf1)-ssRNA-Csn |
Target | RTN4R |
Alternative Names | RTN4R; NGR; NOGOR; Reticulon 4 receptor |
Format | Liquid/lyophilized |
Shipping | It is usually shipped overnight at room temperature in small cardboard boxes. If hot temperatures are expected during transit, they will be shipped in polystyrene containers with gel ice packs. |
Handling Advice | No special measures are necessary. Keep away from food, drink and animal feedingstuffs. |
Storage | Plasmid (lyophilized), short-time storage at Room Temperature, recommended store at -20°C or -80°C ; Plasmid (Liquid), blue ice store at 4°C. |
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